Test Details
Methodology
cobas® HCV (Roche Molecular Systems)
Result Turnaround Time
3 - 5 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Use
This test is used to determine the number of international units (IU) of hepatitis C virus (HCV) RNA per milliliter in serum or plasma in known HCV-positive patients.
Special Instructions
This procedure does not provide serial monitoring; it is intended for one-time use only. If serial monitoring is required, please order Hepatitis C Virus (HCV), Quantitative, Real-time PCR (Graphical) [550070].
Limitations
The quantifiable range of the assay is 15 IU/mL to 100,000,000 IU/mL.
Specimen Requirements
Specimen
Serum or plasma
Volume
2.5 mL
Minimum Volume
700 μL (Note: This volume does not allow for repeat testing.)
Container
Gel-barrier tube, lavender-top (EDTA) tube or plasma preparation tube (PPT™)
Collection Instructions
Centrifuge sample within 24 hours of collection. Transfer serum/plasma to a screw-cap polypropylene transport tube. Ship frozen (preferred). Plasma from a PPT™ can be frozen and shipped in situ. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit a separate frozen specimen for each test requested.
Stability Requirements
| Temperature | Period |
|---|---|
| Room temperature | 24 hours (stability provided by manufacturer or literature reference) |
| Refrigerated | 6 days (stability provided by manufacturer or literature reference) |
| Frozen | 6 weeks (stability provided by manufacturer or literature reference) |
| Freeze/thaw cycles | Stable x4 (stability provided by manufacturer or literature reference) |
Storage Instructions
Freeze (preferred) or refrigerate.
Causes for Rejection
Incorrect anticoagulant; PPT™ not centrifuged
References
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 550080 | HCV RT-PCR, Quant (Non-Graph) | 11011-4 | 550266 | Hepatitis C Quantitation | IU/mL | 11011-4 |
| 550080 | HCV RT-PCR, Quant (Non-Graph) | 11011-4 | 550259 | HCV log10 | log10 IU/mL | 38180-6 |
| 550080 | HCV RT-PCR, Quant (Non-Graph) | 11011-4 | 550307 | Test Information: | 19147-8 | |
| Order Code | 550080 | |||||
| Order Code Name | HCV RT-PCR, Quant (Non-Graph) | |||||
| Order Loinc | 11011-4 | |||||
| Result Code | 550266 | |||||
| Result Code Name | Hepatitis C Quantitation | |||||
| UofM | IU/mL | |||||
| Result LOINC | 11011-4 | |||||
| Order Code | 550080 | |||||
| Order Code Name | HCV RT-PCR, Quant (Non-Graph) | |||||
| Order Loinc | 11011-4 | |||||
| Result Code | 550259 | |||||
| Result Code Name | HCV log10 | |||||
| UofM | log10 IU/mL | |||||
| Result LOINC | 38180-6 | |||||
| Order Code | 550080 | |||||
| Order Code Name | HCV RT-PCR, Quant (Non-Graph) | |||||
| Order Loinc | 11011-4 | |||||
| Result Code | 550307 | |||||
| Result Code Name | Test Information: | |||||
| UofM | ||||||
| Result LOINC | 19147-8 |
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 550265 | HCV RNA (International Units) | 550263 | HCV RNA (International Units) | IU/mL | 11011-4 |
| Reflex 1 | ||||||
| Order Code | 550265 | |||||
| Order Name | HCV RNA (International Units) | |||||
| Result Code | 550263 | |||||
| Result Name | HCV RNA (International Units) | |||||
| UofM | IU/mL | |||||
| Result LOINC | 11011-4 | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 550265 | HCV RNA (International Units) | 550264 | HCV log10 | log10 IU/mL | 38180-6 |
| Reflex 1 | ||||||
| Order Code | 550265 | |||||
| Order Name | HCV RNA (International Units) | |||||
| Result Code | 550264 | |||||
| Result Name | HCV log10 | |||||
| UofM | log10 IU/mL | |||||
| Result LOINC | 38180-6 | |||||