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Hepatitis C Virus (HCV), Quantitative, Real-time PCR (Nongraphical)

CPT: 87522
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  • HCV RNA Quantitation, TaqMan®

Special Instructions

This procedure does not provide serial monitoring; it is intended for one-time use only. If serial monitoring is required, please order test 550070.

Expected Turnaround Time

3 - 5 days

Specimen Requirements


Serum or plasma


2.5 mL

Minimum Volume

700 μL (Note: This volume does not allow for repeat testing.)


Gel-barrier tube, lavender-top (EDTA) tube, or plasma preparation tube (PPT™)


Centrifuge sample within 24 hours of collection. Transfer serum/plasma to a screw-cap polypropylene transport tube. Ship frozen (preferred). Plasma from a PPT™ can be frozen and shipped in situ. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit a separate frozen specimen for each test requested.

Storage Instructions

Freeze (preferred) or refrigerate. Stable at room temperature for 24 hours or refrigerated for 72 hours. Stable frozen for six weeks.

Causes for Rejection

Incorrect anticoagulant; PPT™ not centrifuged

Test Details


Determine the number of international units (IU) of hepatitis C virus (HCV) RNA per milliliter in serum or plasma in known HCV-positive patients


The quantifiable range of the assay is 15 IU/mL to 100,000,000 IU/mL.


Cobas® AmpliPrep/Cobas® TaqMan® HCV Test v 2.0


Kleiber J, Walter T, Haberhausen G, Tsang S, Babiel R, Rosenstraus M. Performance characteristics of a quantitative, homogeneous TaqMan™ RT-PCR test for HCV RNA. J Mol Diagn. 2000 Aug; 2(3):158-166. 11229521


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
550080 HCV RT-PCR, Quant (Non-Graph) 11011-4 550266 Hepatitis C Quantitation IU/mL 11011-4
550080 HCV RT-PCR, Quant (Non-Graph) 11011-4 550259 HCV log10 log10 IU/mL 38180-6
550080 HCV RT-PCR, Quant (Non-Graph) 11011-4 550307 Test Information: N/A
Reflex Table for Hepatitis C Quantitation
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550265 HCV RNA (International Units) 550263 HCV RNA (International Units) IU/mL 11011-4
Reflex Table for Hepatitis C Quantitation
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 550265 HCV RNA (International Units) 550264 HCV log10 log10 IU/mL 38180-6

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