Test Details
Methodology
Chemiluminescence Immunoassay
Result Turnaround Time
10 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Use
This test is one measurement of anti-Ro60 antibodies by chemiluminescent immunoassay. Anti-Ro occur in 60% to 90% of patients with Sjögren’s Syndrome (SS), in 30% to 50% of patients with systemic lupus erythematosus (SLE) and less frequently in other autoimmune diseases. Historically, autoantibodies to both parts of the Ro (SS-A) antigen, Ro52 kilodaltons and Ro60 kilodaltons, have been used to comprise total anti-Ro. Now it is known that Ro52 and Ro60 proteins are not linked in the same particle. In addition, anti-Ro52 and anti-Ro60 are distinct from each other and have different clinical utilities. Ro60 is a protein component of small cytoplasmic RNA complexes that bind misfolded, non-coding RNA. Ro60 may be involved in surveillance of defective RNA. Isolated anti-Ro60 positivity is associated most frequently with SLE and less commonly with Sjogren’s, rheumatoid arthritis, systemic sclerosis, idiopathic inflammatory myopathy (IIM), overlap and other autoimmune diseases.
Limitations
This anti-Ro60 test is performed by chemiluminescent immunoassay and its numeric results cannot be used additively with measurements of Anti-Ro52 by enzyme immunoassay. The sum of the two measurements do not equate to total Ro antibodies. Results should be interpreted in the context of other laboratory and clinical findings. Negative results do not exclude the possibility of autoimmune disease.
Specimen Requirements
Specimen
Serum
Volume
1 mL
Minimum Volume
0.5 mL (Note: This volume does not allow for repeat testing.)
Container
Gel-barrier tube, red-top tube
Collection Instructions
If a red-top tube is used, transfer separated serum to a plastic transport tube.
Stability Requirements
Room temperature: 48 hours (stability provided by manufacturer or literature reference)
Refrigerated: 14 days (stability provided by manufacturer or literature reference)
Frozen: 14 days (stability provided by manufacturer or literature reference)
Freeze/thaw cycles: Stable x3 (stability provided by manufacturer or literature reference)
Storage Instructions
Refrigerate or freeze.
Causes for Rejection
Gross hemolysis, lipemia and icterus
References
Alexander EL, Hirsch TJ, Arnett FC, Provost TT, Stevens MB. Ro(SS-A) and La(SS-B) antibodies in the clinical spectrum of Sjogren's Syndrome. J Rheumatol. 1982 Mar-Apr;9(2):239-246. PubMed 7097683
Alspaugh MS, Talal N, Tan EM. Differentiation and characterization of a autoantibodies and their antigens in Sjogren's Syndrome. Arthritis Rheum. 1976 Mar-Apr;19(2):216-222. PubMed 1259795
Egner W. The use of laboratory tests in the diagnosis of SLE. J Clin Pathol. 2000 Jun;53(6):424-432. PubMed 10911799
Friedman DM, Rupel A, Buyon JP. Epidemiology, etiology, detection, and treatment of autoantibody-associate congenital heart block in neonatal lupus. Curr Rheumatol Rep. 2007 May;9(2):101-108. PubMed 17502039
Madison PJ, Provost TT, Reichlin M. Serologic findings in patients with "ANA negative" systemic lupus erythematosus. Medicine (Baltimore). 1981 Mar;60(2):87-94. PubMed 6971388
Schulte-Pelkum J, Fritzler M, Mahler M. Latest update on the Ro-SS-A autoantibody system. Autoimmune Rev. 2009 Jun;8(7):632-637. PubMed 19393201
Shiboski SC, Shiboski CH, Criswell LA, et al. American College of Rheumatology Classification Criteria for Sjogren's Syndrome: A Data-Driven, Expert Consensus Approach in the Sjogren's International Collaborative Clinical Alliance Cohort. Arthritis Care Res (Hoboken). 2012 Apr;64(4):475-487. PubMed 22563590
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 505785 | Anti-Ro60 (SS-A 60 kD) | 505787 | Anti-Ro60 (SS-A 60 kD) | CU | Pending | |
| Order Code | 505785 | |||||
| Order Code Name | Anti-Ro60 (SS-A 60 kD) | |||||
| Order Loinc | ||||||
| Result Code | 505787 | |||||
| Result Code Name | Anti-Ro60 (SS-A 60 kD) | |||||
| UofM | CU | |||||
| Result LOINC | Pending |