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Please provide a copy of the pathology report. IDH1/2 testing will be delayed if the pathology report is not received. Please direct any questions regarding this test to customer service at 800-345-4363.
5 - 7 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Whole blood, bone marrow, formalin-fixed, paraffin-embedded (FFPE) tissue block or slides
3 to 5 mL whole blood, 1 to 2 mL bone marrow, four pre-cut unstained slides at 5 micron with one matching H&E reference slide, or formalin-fixed, paraffin-embedded tissue (FFPE) block
3 mL whole blood, 1 mL bone marrow, two unstained slides at 5µm and one matching H&E slide, or three unstained slides at 5µm. Tumor surface area >=4mm2 tumor area and >/= 50% tumor content are preferred
Lavender-top (EDTA) tube, green-top (heparin) tube, yellow-top (ACD) tube, FFPE tissue block and slide container
Refrigerate. Maintain FFPE blocks/slides at room temperature.
Specimen does not meet all of the above criteria for sample type, container, minimum volume, collection and storage; unsuitable specimens include but are not limited to: frozen whole blood or marrow; a leaking tube; clotted blood or marrow; a grossly hemolyzed specimen or otherwise visibly degraded; specimen suspected of being contaminated by another specimen; specimen contains suspicious foreign material; no tumor tissue in FFPE block or slides; broken or stained slides
Isocitrate dehydrogenase 1 and 2 (IDH1 and IDH2) are the most frequently mutated metabolic genes in human cancer. They encode cytosolic and mitochondrial enzymes that catalyze the conversion of isocitrate to a-ketoglutarate (aKG), a key component in metabolic and cellular pathways including the Krebs cycle. IDH1 and IDH2 mutations are found in multiple types of human cancer including, but not limited to, acute myeloid leukemia and gliomas. Identification of IDH mutations can aid in their diagnosis, provide prognostic information, and suggest treatment with IDH inhibitors. This assay will detect mutations affecting amino acids 100, 105, and 132 of IDH1, and amino acids 140 and 172 of IDH2.
In vitro studies indicate that this assay has a sensitivity to detect approximately 5% mutated IDH1/2 in a background of non-mutant DNA. Mutations present at a level below the detection sensitivity or outside the analyzed region of the IDH1 and IDH2 genes will not be detected by this assay.
This test was developed, and its performance characteristics determined, by LabCorp. It has not been cleared or approved by the US Food and Drug Administration (FDA).
SNaPshot Multiplex PCR (primer extension-based method)
|Order Code||Order Code Name||Order Loinc||Result Code||Result Code Name||UofM||Result LOINC|
|481484||IDH1/IDH2 Mutation Analysis||481485||IDH1 Result||50397-9|
|481484||IDH1/IDH2 Mutation Analysis||481486||IDH1 Nuc Change||48004-6|
|481484||IDH1/IDH2 Mutation Analysis||481487||IDH1 Amino Acid Change||48005-3|
|481484||IDH1/IDH2 Mutation Analysis||481488||IDH2 Result||50397-9|
|481484||IDH1/IDH2 Mutation Analysis||481489||IDH2 Nuc Change||48004-6|
|481484||IDH1/IDH2 Mutation Analysis||481490||IDH2 Amino Acid Change||48005-3|
|481484||IDH1/IDH2 Mutation Analysis||481491||Indication||42349-1|
|481484||IDH1/IDH2 Mutation Analysis||481492||Location||n/a|
|481484||IDH1/IDH2 Mutation Analysis||481493||Specimen Type||31208-2|
|481484||IDH1/IDH2 Mutation Analysis||481494||Block ID||57723-9|
|481484||IDH1/IDH2 Mutation Analysis||481495||Background||8251-1|
|481484||IDH1/IDH2 Mutation Analysis||481979||Method||49549-9|
|481484||IDH1/IDH2 Mutation Analysis||481497||References||75608-0|
|481484||IDH1/IDH2 Mutation Analysis||481498||Director Review||72486-4|
|481484||IDH1/IDH2 Mutation Analysis||480903||Microdissection Performed||8100-0|
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