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This assay is currently not available in New York state.
Please provide a copy of the pathology report. Sample submission without a copy of the pathology report may delay testing. Please direct any questions regarding this test to customer service at 800-345-4363.
7 - 10 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
For more information, please view the literature below.
Formalin-fixed, paraffin-embedded (FFPE) tissue blocks or slides.
FFPE tissue block or four unstained slides at 5 μM and one matching H&E slide or five precut unstained slides from a paraffin block in 5-μM sections.
Two unstained slides at 5 μM and one matching H&E slide or three unstained slides at 5 μM (Note: Samples with >4mm2 and >/= 50% tumor content are preferred.)
FFPE tissue block or four unstained slides at 5 μM and one matching H&E slide or five precut unstained slides from a paraffin block in 5-μM sections
Maintain blocks/slides at room temperature.
Tissue is not primary brain tumor; tumor block containing insufficient tumor tissue (<4mm2); tumor fixed in a heavy metal fixative; broken or stained slides; decalcified specimens
Tissue is not primary brain tumor; tumor block containing insufficient tumor tissue (<4mm2); tumor fixed in a heavy
Tissue is not primary brain tumor; tumor block containing insufficient tumor tissue (<4mm2); tumor fixed in a heavy heavy metal fixative; broken or stained slides; decalcified specimens
MGMT (O6-methylguanine-DNA methyltransferase) is a DNA repair enzyme that is involved in the repair of damage caused by a variety DNA cross-linking compounds, including alkylating agents. Increased methylation of the MGMT gene promoter region causes diminished or silenced expression of the gene, making cells more sensitive to DNA damage. The relationship has been shown for glioblastomas and alkylating agents such as temozolomide (Temodar®).
Preparation of DNA from tissue samples is dependent upon the quality of the provided specimen. Inadequate DNA extraction may occur in a significant number of paraffin-embedded samples.
Quantitative methylation-specific polymerase chain reaction (PCR)
Approximately 40% to 50% of glioblastoma multiforme (GBM) tumors exhibit MGMT gene methylation. Retrospective studies have shown that detection of MGMT promoter methylation in tumor samples is associated with an increased likelihood of a favorable response to temozolomide.
|Order Code||Order Code Name||Order Loinc||Result Code||Result Code Name||UofM||Result LOINC|
|489280||MGMT Methylation Assay||489281||Result:||60252-4|
|489280||MGMT Methylation Assay||489268||Methylation Score:||64083-9|
|489280||MGMT Methylation Assay||489283||Block Number:||N/A|
|489280||MGMT Methylation Assay||489284||Background:||77202-0|
|489280||MGMT Methylation Assay||489299||Methodology:||49549-9|
|489280||MGMT Methylation Assay||489286||References:||N/A|
|489280||MGMT Methylation Assay||489282||Director Review:||72486-4|
|489280||MGMT Methylation Assay||480903||Microdissection Performed||8100-0|
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