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GeneSeq® Neuro Single Gene, Fetal Analysis

CPT

To be determined. Updates will be made when available.

Synonyms

Prenatal full gene sequencing; prenatal gene specific sequencing

Test Details

Methodology

Next-generation sequencing to identify genetic variants, including single nucleotide variants (SNVs), insertions, deletions and copy number variants (CNVs)

Result Turnaround Time

28 days; in some cases, additional time may be required for confirmatory or reflex tests. If culture is needed, an additional 14-21 days may be required. Additional culture fee may be applied. 

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Test Includes

This test includes the following genes: AARS1, ABHD12, ADAMTS2, AEBP1, AGA, AIFM1, ANO5, ARHGEF10, ARSA, ARSB, ASAH1, ATL1, ATL3, ATP13A2, ATP1A1, ATP7A, B3GALT6, B4GALT7, BAG3, BICD2, BSCL2, BVES, C1R, C1S, CACNA1S, CADM3, CAPN3, CAV3, CHCHD10, CHST14, CLCF1, CLN3, CLN5, CLN6, CLN8, CNTNAP1, COA7, COL12A1, COL1A1, COL1A2, COL3A1, COL5A1, COL5A2, COL6A1, COL6A2, COL6A3, COX6A1, CRLF1, CRPPA, CTDP1, CTNS, CTSA, CTSD, CTSF, CTSK, DAG1, DCAF8, DCTN1, DES, DGAT2, DHTKD1, DNAJB2, DNAJB6, DNAJC5, DNM2, DNMT1, DRP2, DSE, DST, DYNC1H1, DYSF, EGR2, ELP1, EMD, FBLN5, FBXO38, FGD4, FHL1, FIG4, FKBP14, FKRP, FKTN, FLVCR1, FUCA1, GAA, GALC, GALNS, GAN, GARS1, GBA1, GBF1, GDAP1, GJB1, GLA, GLB1, GM2A, GMPPB, GNB4, GNE, GNPTAB, GNPTG, GNS, GRN, GUSB, HARS1, HEXA, HEXB, HGSNAT, HINT1, HK1, HNRNPDL, HSPB1, HSPB8, HTRA1, HYAL1, IDS, IDUA, IGHMBP2, INF2, JAG2, KARS1, KCNJ2, KCNJ5, KCTD7, KIF1A, KIF1B, KIF5A, LAMA2, LAMP2, LIPA, LITAF, LMBRD1, LMNA, LRRK2, LRSAM1, LYST, MAN2B1, MANBA, MARS1, MCM3AP, MCOLN1, MECP2, MED25, MEFV, MFN2, MFSD8, MME, MORC2, MPV17, MPZ, MTMR2, MTRFR, MVK, MYH14, MYOT, NAGA, NAGLU, NDRG1, NEFH, NEFL, NEU1, NF2, NGF, NLRC4, NLRP3, NOTCH3, NPC1, NPC2, NTRK1, PDK3, PIEZO2, PLEC, PLEKHG5, PLOD1, PMP2, PMP22, PNKP, POGLUT1, POMGNT1, POMGNT2, POMT1, POMT2, POPDC3, PPT1, PRDM12, PRDM5, PRPS1, PRX, PSAP, PTRH2, RAB7A, REEP1, RETREG1, RYR1, SACS, SBF1, SBF2, SCARB2, SCN10A, SCN11A, SCN4A, SCN9A, SCO2, SEPTIN9, SETX, SGCA, SGCB, SGCD, SGCG, SGPL1, SGSH, SH3TC2, SIGMAR1, SLC12A6, SLC17A5, SLC25A46, SLC39A13, SLC5A7, SMPD1, SOD1, SORD, SPG11, SPTLC1, SPTLC2, STAC3, SUMF1, SURF1, SYNE1, SYNE2, TCAP, TECPR2, TFG, TMEM43, TNFRSF1A, TNPO3, TNXB, TOR1AIP1, TPP1, TRAPPC11, TRIM2, TRIM32, TRPA1, TRPV4, TSC1, TSC2, TTN, TTR, TUBB3, VCP, VPS16, VPS33A, VWA1, WARS1, WNK1, YARS1, ZFHX2 and ZNF469.

Use

This test is used for prenatal diagnosis for pregnancies at risk for any of the genes listed in the Test Includes section.  

Special Instructions

All clients should call 844-664-8378 (844-MNGTEST) to speak with a genetic coordinator before collecting the specimen. The specific gene(s) to be analyzed must be indicated on the test requisition form. Failure to indicate the gene(s) will result in testing delays. All fetal specimens, including cord blood, must be accompanied by a maternal blood, PurFlock buccal swab kit or Oragene Dx 500 saliva kit for maternal cell contamination (MCC). A separate requisition should be submitted with the maternal specimen.

Limitations

This assay does not consistently detect mosaicism or rule out the presence of large chromosomal aberrations, including rearrangements and inversions that do not change copy number of genomic regions. This NGS assay does not detect repeat expansions. False positive or false negative results may occur for reasons that include insufficient information available about rare genetic variants, sex chromosome abnormalities, pseudogene interference, homologous regions, blood transfusions, bone marrow transplantation, somatic or tissue-specific mosaicism, mislabeled samples or erroneous representation of family relationships. Interpretation of the clinical significance of gene variations is limited by information about the variant available at the time of reporting and by the quality and quantity of clinical information provided with the sample. The interpretation of the clinical significance of variants may change.

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.
 

Specimen Requirements

Specimen

Amniotic fluid; chorionic villus sample (CVS); cultured cells or cord blood. Direct amniotic fluid or CVS specimen may be submitted; additional culture fee may be applied.

Volume

Amniotic fluid: 20 mL; CVS: 20mg; amniotic fluid and CVS culture: two confluent T-25 flasks; or cord blood: 4 mL (if amniotic fluid or CVS are cultured at another facility, please maintain back-up cultures)
 

Minimum Volume

Amniotic fluid: 20 mL; CVS culture: 20mg; amniotic fluid and CVS culture: two confluent T-25 flasks; or cord blood: 3 mL (if amniotic fluid or CVS are cultured at another facility, please maintain back-up cultures)
 

Container

Amniotic fluid or CVS: sterile plastic conical tube or T-25 flask; cord blood: lavender-top (EDTA) 

Collection Instructions

Standard sterile techniques; transfer aseptically to sterile tubes. 

Amniotic fluid: Discard first 2 mL of fluid aspirated to avoid maternal cell contamination. 

Stability Requirements

Please ship expedited at room temperature.

Storage Instructions

Maintain specimen at room temperature. Do not freeze.

Causes for Rejection

Frozen or hemolyzed specimen; quantity not sufficient for analysis; improper container

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
631148 Neuro Single Gene, Fetal 631207 Extraction N/A
631148 Neuro Single Gene, Fetal 631208 Billing Hold N/A
631148 Neuro Single Gene, Fetal 631209 Result 50397-9
631148 Neuro Single Gene, Fetal 631210 Interpretation 93364-8
631148 Neuro Single Gene, Fetal 631211 Footnotes 55752-0
631148 Neuro Single Gene, Fetal 631212 PDF 51969-4
Order Code631148
Order Code NameNeuro Single Gene, Fetal
Order Loinc
Result Code631207
Result Code NameExtraction
UofM
Result LOINCN/A
Order Code631148
Order Code NameNeuro Single Gene, Fetal
Order Loinc
Result Code631208
Result Code NameBilling Hold
UofM
Result LOINCN/A
Order Code631148
Order Code NameNeuro Single Gene, Fetal
Order Loinc
Result Code631209
Result Code NameResult
UofM
Result LOINC50397-9
Order Code631148
Order Code NameNeuro Single Gene, Fetal
Order Loinc
Result Code631210
Result Code NameInterpretation
UofM
Result LOINC93364-8
Order Code631148
Order Code NameNeuro Single Gene, Fetal
Order Loinc
Result Code631211
Result Code NameFootnotes
UofM
Result LOINC55752-0
Order Code631148
Order Code NameNeuro Single Gene, Fetal
Order Loinc
Result Code631212
Result Code NamePDF
UofM
Result LOINC51969-4