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3-O-Methyldopa (Plasma)

CPT 82131

Test Details

Methodology

Liquid chromatography/tandem mass spectrometry (LC/MS-MS)

Result Turnaround Time

10 - 14 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Use

Aromatic L-amino acid decarboxylase (AADC) is a pyridoxal 5'-phosphate dependent enzyme responsible for the formation of dopamine and serotonin. AADC deficiency is a congenital autosomal recessive metabolic disorder that causes hypotonia, hypokinesia, oculogyric crises, and signs of autonomic dysfunction beginning in infancy. In AADC deficiency, L-dopa accumulates and can be methylated by catechol O-methyltransferase using S-adenosylmethionine as the methyl donor to form 3-O-methyldopa (3-OMD). 3-OMD is more stable than L-dopa and can be detected in blood.

Limitations

This test was developed and its performance characteristics determined by LabCorp. It has not been cleared or approved by the Food and Drug Administration.

Specimen Requirements

Specimen

Plasma, frozen

Volume

1.0 mL

Minimum Volume

0.5 mL

Container

Lavender-top (EDTA) tube, green-top (sodium heparin) tube

Collection Instructions

Collect 2-4 mL blood in sodium heparin (green-top) or EDTA (purple-top) tube.

Stability Requirements

Temperature

Period

Room temperature

Unstable

Refrigerated

Unstable

Frozen

Indefinitely

Storage Instructions

Remove plasma and freeze at -20°C. Store frozen at -20°C and ship frozen.

Causes for Rejection

Thawed sample

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
620176 3-O-Methyldopa Plasma 50141-1 620177 Result 50141-1
620176 3-O-Methyldopa Plasma 50141-1 620178 Interpretation N/A
620176 3-O-Methyldopa Plasma 50141-1 630857 PDF 80563-0
Order Code620176
Order Code Name3-O-Methyldopa Plasma
Order Loinc50141-1
Result Code620177
Result Code NameResult
UofM
Result LOINC50141-1
Order Code620176
Order Code Name3-O-Methyldopa Plasma
Order Loinc50141-1
Result Code620178
Result Code NameInterpretation
UofM
Result LOINCN/A
Order Code620176
Order Code Name3-O-Methyldopa Plasma
Order Loinc50141-1
Result Code630857
Result Code NamePDF
UofM
Result LOINC80563-0