Human Immunodeficiency Virus 1 (HIV-1), Quantitative, RNA (Abbott® RealTime)

CPT: 87536
Updated on 03/26/2024
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Synonyms

  • HIV-1 Plasma Viremia
  • HIV-1 RNA by Real-time (RT) PCR, Quantitative
  • Viral Load, Abbott, Real-time

Expected Turnaround Time

3 - 7 days



Related Documents


Specimen Requirements


Specimen

Plasma, frozen


Volume

2 mL

3.5 mL

2 mL


Minimum Volume

1.1 mL (Note: This volume does not allow for repeat testing.)


Container

Ship in a screw-cap polypropylene frozen transport tube or centrifuged PPT™ tubes.


Collection

Collect specimen in lavender-top (EDTA), PPT™, or yellow-top (ACD-A) tubes. Centrifuge specimen. If drawn in EDTA or ACD, the plasma must be transferred to a screw-cap polypropylene transport tube and frozen. If refrigerated, whole blood specimen may be held for 48 hours prior to centrifugation. If room temperature, whole blood specimen may be held for 24 hours prior to centrifugation. Plasma specimens may be stored at -20°C for 60 days. If longer storage is required, plasma specimens may be stored at -70°C or lower for 6 months. Ship frozen on dry ice. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Collect specimen in lavender-top (EDTA), PPT™, or yellow-top (ACD-A) tubes. Centrifuge specimen within six hours of collection when freshly drawn at 15°C to 30°C. If drawn in EDTA or ACD, the plasma must be transferred to a screw-cap polypropylene transport tube and frozen. If refrigerated, specimen may be held for 24 hours prior to centrifugation. Plasma specimens may be stored at -20°C for 60 days. If longer storage is required, plasma specimens must be stored at -70°C or lower. Ship frozen on dry ice. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Collect specimen in lavender-top (EDTA), PPT™, or yellow-top (ACD-A) tubes. Centrifuge specimen. If drawn in EDTA or ACD, the plasma must be transferred to a screw-cap polypropylene transport tube and frozen. If refrigerated, whole blood specimen may be held for 48 hours prior to centrifugation. If room temperature, whole blood specimen may be held for 24 hours prior to centrifugation. Plasma specimens may be stored at -20°C for 60 days. If longer storage is required, plasma specimens may be stored at -70°C or lower for 6 months. Ship frozen on dry ice. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.


Storage Instructions

Frozen (preferred)

Freeze; stable for long-term storage. Stable at room temperature for six hours or one day after centrifugation. Stable refrigerated for 24 hours or five days after centrifugation.

Frozen (preferred)


Stability Requirements

Temperature

Period

Room temperature

24 hours

Refrigerated

72 hours

Frozen

-20°C = 60 days

-70°C = 6 months

Freeze/thaw cycles

Stable x0

Temperature

Period

Room temperature

24 hours

Refrigerated

72 hours

Frozen

-20°C = 60 days

-70°C = 6 months

Freeze/thaw cycles

Stable x0


Causes for Rejection

Hemolysis; plasma other than EDTA or ACD-A; quantity not sufficient for analysis


Test Details


Use

Detect and quantitate HIV-1 in plasma


Limitations

Assay range: 20−10,000,000 copies/mL

Assay range: 40−10,000,000 copies/mL

Assay range: 20−10,000,000 copies/mL


Methodology

Abbott Alinity m HIV-1 assay

Abbott m2000 RealTime HIV-1 assay

Abbott Alinity m HIV-1 assay


References

Huang S, Salituro J, Tang N, et al. Thermodynamically modulated partially double-stranded linear DNA probe design for homogeneous real-time PCR. Nucleic Acids Res. 2007; 35(16):e101.17693434
Tang N, Huang S, Salituro J, et al. A RealTime HIV-1 viral load assay for automated quantitation of HIV-1 RNA in genetically diverse group M subtypes A-H, group O and group N samples. J Virol Methods. 2007; 146(1-2):236-245. 17707519

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
550880 RealTime Abbott 75622-1 550881 HIV-1 RNA by PCR copies/mL 20447-9
550880 RealTime Abbott 75622-1 550882 log10 HIV-1 RNA log10copy/mL 29541-0

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