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Human Immunodeficiency Virus 1 (HIV-1), Quantitative, RNA (Abbott® RealTime)

CPT: 87536
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Synonyms

  • HIV-1 Plasma Viremia
  • HIV-1 RNA by Real-time (RT) PCR, Quantitative
  • Viral Load, Abbott, Real-time

Expected Turnaround Time

3 - 7 days



Related Documents


Specimen Requirements


Specimen

Plasma, frozen


Volume

3.5 mL


Minimum Volume

1.1 mL (Note: This volume does not allow for repeat testing.)


Container

Ship in a screw-cap polypropylene frozen transport tube or centrifuged PPT™ tubes.


Collection

Collect specimen in two lavender-top (EDTA), PPT™, or yellow-top (ACD-A) tubes. Centrifuge specimen within six hours of collection when freshly drawn at 15°C to 30°C. If drawn in EDTA or ACD, the plasma must be transferred to a screw-cap polypropylene transport tube and frozen. If refrigerated, specimen may be held for 24 hours prior to centrifugation. Plasma specimens may be stored at -20°C for 60 days. If longer storage is required, plasma specimens must be stored at -70°C or lower. Ship frozen on dry ice. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.


Storage Instructions

Freeze; stable for long-term storage. Stable at room temperature for six hours or one day after centrifugation. Stable refrigerated for 24 hours or five days after centrifugation.


Causes for Rejection

Hemolysis; plasma other than EDTA or ACD-A; quantity not sufficient for analysis


Test Details


Use

Detect and quantitate HIV-1 in plasma


Limitations

Assay range: 40−10,000,000 copies/mL


Methodology

Abbott m2000 RealTime HIV-1 assay


References

Huang S, Salituro J, Tang N, et al. Thermodynamically modulated partially double-stranded linear DNA probe design for homogeneous real-time PCR. Nucleic Acids Res. 2007; 35(16):e101.17693434
Tang N, Huang S, Salituro J, et al. A RealTime HIV-1 viral load assay for automated quantitation of HIV-1 RNA in genetically diverse group M subtypes A-H, group O and group N samples. J Virol Methods. 2007; 146(1-2):236-245. 17707519

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
550880 RealTime Abbott 550881 HIV-1 RNA by PCR copies/mL 20447-9
550880 RealTime Abbott 550882 log10 HIV-1 RNA log10copy/mL 29541-0

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