Human Immunodeficiency Virus 1 (HIV-1), Quantitative, Real-time PCR (Nongraphical) With Reflex to HIV-1 GenoSure® MG

CPT: 87536
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Synonyms

  • HIV-1 Plasma Viremia
  • HIV-1 RNA by Real-time PCR, Quantitative
  • Reflex to Genotype
  • Viral Load

Test Includes

Reflex to genotype (at additional charge) if viral load is >500 copies/mL


Special Instructions

This procedure does not provide serial monitoring; it is intended for one-time use only. If serial monitoring is required, please order test 550422.


Expected Turnaround Time

5 - 13 days




Specimen Requirements


Specimen

Plasma, frozen


Volume

6 mL


Minimum Volume

4 mL (Note: This volume does not allow for repeat testing.)


Container

Ship in a screw-cap polypropylene frozen transport tube.


Collection

Collect specimen in three lavender-top (EDTA) tubes or three PPT™ tubes. Do not use green-top (heparin) tubes. Centrifuge specimen within six hours of collection. Transfer plasma to one or more screw-cap polypropylene transport tube(s). "Snap-cap" or "pop-top" tubes should be avoided and may result in specimen rejection. Immediately freeze specimen at -20°C. Do not place samples on dry ice until completely frozen. Ship specimens frozen. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.


Storage Instructions

Freeze. Stable at room temperature for one day or refrigerated for five days. Freeze for long-term storage.


Causes for Rejection

Hemolysis; green-top (heparin) tube; quantity not sufficient for analysis; frozen PPT™


Test Details


Use

Detect and quantitate HIV-1 in plasma with reflex to genotype (at additional charge) if viral load is >500 copies/mL. GenoSure® MG test detects mutations in the HIV viral genes that encode the HIV protease and polymerase (reverse transcriptase). Mutations in these genes have been associated with resistance to antiretroviral drugs.


Limitations

Assay range: 20−10,000,000 copies/mL


Methodology

Cobas® AmpliPrep/Cobas® TaqMan® HIV-1 Test, Version 2.0


LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
550432 RNA, PCR (NonGraph) rfx/Geno 551308 HIV-1 RNA by PCR copies/mL 20447-9
550432 RNA, PCR (NonGraph) rfx/Geno 551314 log10 HIV-1 RNA log10copy/mL 29541-0
550432 RNA, PCR (NonGraph) rfx/Geno 551272 Genotype Assay N/A
Reflex Table for Genotype Assay
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 551568 Reflex to GenoSure(R) MG 551693 PDF 49573-9
Reflex Table for Genotype Assay
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 551568 Reflex to GenoSure(R) MG 551656 HIV GenoSure(R) 93047-9
Reflex 2 551318 INSUF4 551311 HIV-1 RNA by PCR copies/mL 20447-9
Reflex Table for Genotype Assay
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 551568 Reflex to GenoSure(R) MG 551656 HIV GenoSure(R) 93047-9
Reflex 2 551318 INSUF4 551312 log10 HIV-1 RNA log10copy/mL 29541-0
Reflex Table for Genotype Assay
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 551568 Reflex to GenoSure(R) MG 551656 HIV GenoSure(R) 93047-9
Reflex 2 551624 PGAAS 551624 HIV GenoSure(R) MG 49573-9
Reflex Table for Genotype Assay
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 551568 Reflex to GenoSure(R) MG 551657 HIV GenoSure(R) 53798-5
Reflex 2 551619 MGSURE 551619 HIV GenoSure(R) MG Interp N/A
Reflex Table for Genotype Assay
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 551568 Reflex to GenoSure(R) MG 551566 Tracking N/A

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