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Anti-SS-A 52kD Ab, IgG (RDL)

CPT: 86235

Synonyms

  • Topoisomerase 1

Test Includes

This test is a component of Interstitial Lung Disease Panel I, Interstitial Lung Disease Panel II, ILdx Complete, MyoMarker 3 Plus Profile, MyoMarker 3 Profile.


Expected Turnaround Time

7 - 14 days


Specimen Requirements


Specimen

Serum


Volume

2 mL


Minimum Volume

0.5 mL (Note: This volume does not allow for repeat testing.)


Container

Red-top tube or gel-barrier tube


Collection

Separate serum from cells within one hour of collection. Transfer to a plastic transport tube before shipping. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.


Storage Instructions

Refrigerate or freeze.


Stability Requirements

Temperature

Period

Room temperature

7 days

Refrigerated

14 days

Frozen

60 days

Freeze/thaw cycles

Stable x1


Causes for Rejection

Grossly hemolyzed; bacterial contamination; lipemic specimen; icteric specimen; non-serum specimen types


Test Details


Use

The presence of these antibodies, when considered in conjunction with other laboratory and clinical findings, is an aid in the diagnosis of systemic lupus erythematosus (SLE), Sjögren’s Syndrome (SS), systemic sclerosis (SSC), polymyositis (PM) and dermatomyositis (DM). SS-A/Ro antigen are found in 40-60% of patients withSjögren’s Syndrome and in 25-35% of patients with SLE. At one time it was thought that two different proteins, SS-A 52 and SS-A 60, were needed to account for SS-A antigenic reactivity. Now it is known that SS-A 52 and SS-A 60 proteins are not linked in the same particle. In addition, anti-SS-A 52 autoantibodies are distinct from, and have a different clinical utility than, anti-SS-A 60 antibodies. Just like anti-SS-A 60, anti-SS-A 52 is commonly found in patients with SLE and Sjgren's Syndrome, and occasionally in other autoimmune disorders. However, anti-SS-A 52 autoantibodies are also found in patients with systemic sclerosis, polymyositis and dermatomyositis. Anti-SSA 52 are linked with antibodies to tRNA synthetases like Jo-1, PL-7, PL-12, EJ and OJ, which are often found in patients with interstitial lung involvement. Studies show that 10-45% of myositis is positive with SSA52 antibody. An RDL study revealed 38% of myositis patients (18/47) were positive for SS-A 52 antibody. RDL offers SS-A 52 antibody test as part of the myositis panel.


Methodology

Enzyme-linked immunosorbent assay (ELISA)


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CPT Statement/Profile Statement

The LOINC® codes are copyright © 1994-2020, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf