Test Details
Methodology
Fluorescence in situ hybridization (FISH)
Result Turnaround Time
3 - 5 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Test Includes
Fluorescence in situ hybridization (FISH) for myeloproliferative neoplasms/chronic myelogenous leukemia (MPN/CML) targeting numerical changes of chromosomes 8, 9, 13q14, and rearrangement of BCR/ABL1.
Use
Confirmation/identification of chromosome abnormalities in interphase nuclei. Leukemia monitoring of residual disease.
Special Instructions
Include the pertinent clinical diagnosis, previous cytogenetic studies, and probe of interest with the test request form.
Specimen Requirements
Specimen
Bone marrow, blood, cerebrospinal fluid (CSF), FNA, bone marrow touch prep slides
Volume
3 mL bone marrow (For additional cytogenetic studies an additional 1 mL of bone marrow is required.), fixed-cell pellet, three slides
Minimum Volume
Fixed-cell pellet from a cytogenetic analysis, five slides, 2 mL blood, or 1 mL bone marrow (For additional cytogenetic studies, an additional 1 mL of bone marrow is required.)
Container
Green-top (heparin) tube; pediatric Vacutainer® is optimal or lavender-top (EDTA) tube
Storage Instructions
Maintain specimens at room temperature.
Causes for Rejection
Broken or dirty slides; excessive cellular debris; stains on slides
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 511425 | MPN/CML FISH | 511087 | Specimen Type | 31208-2 | ||
| 511425 | MPN/CML FISH | 511088 | Cells Counted | 62361-1 | ||
| 511425 | MPN/CML FISH | 511091 | Cells Analyzed | 62360-3 | ||
| 511425 | MPN/CML FISH | 511092 | FISH Result | 62356-1 | ||
| 511425 | MPN/CML FISH | 511093 | Interpretation | 62365-2 | ||
| 511425 | MPN/CML FISH | 511094 | Director Review: | 48672-0 | ||
| 511425 | MPN/CML FISH | 512123 | 11502-2 | |||
| Order Code | 511425 | |||||
| Order Code Name | MPN/CML FISH | |||||
| Order Loinc | ||||||
| Result Code | 511087 | |||||
| Result Code Name | Specimen Type | |||||
| UofM | ||||||
| Result LOINC | 31208-2 | |||||
| Order Code | 511425 | |||||
| Order Code Name | MPN/CML FISH | |||||
| Order Loinc | ||||||
| Result Code | 511088 | |||||
| Result Code Name | Cells Counted | |||||
| UofM | ||||||
| Result LOINC | 62361-1 | |||||
| Order Code | 511425 | |||||
| Order Code Name | MPN/CML FISH | |||||
| Order Loinc | ||||||
| Result Code | 511091 | |||||
| Result Code Name | Cells Analyzed | |||||
| UofM | ||||||
| Result LOINC | 62360-3 | |||||
| Order Code | 511425 | |||||
| Order Code Name | MPN/CML FISH | |||||
| Order Loinc | ||||||
| Result Code | 511092 | |||||
| Result Code Name | FISH Result | |||||
| UofM | ||||||
| Result LOINC | 62356-1 | |||||
| Order Code | 511425 | |||||
| Order Code Name | MPN/CML FISH | |||||
| Order Loinc | ||||||
| Result Code | 511093 | |||||
| Result Code Name | Interpretation | |||||
| UofM | ||||||
| Result LOINC | 62365-2 | |||||
| Order Code | 511425 | |||||
| Order Code Name | MPN/CML FISH | |||||
| Order Loinc | ||||||
| Result Code | 511094 | |||||
| Result Code Name | Director Review: | |||||
| UofM | ||||||
| Result LOINC | 48672-0 | |||||
| Order Code | 511425 | |||||
| Order Code Name | MPN/CML FISH | |||||
| Order Loinc | ||||||
| Result Code | 512123 | |||||
| Result Code Name | ||||||
| UofM | ||||||
| Result LOINC | 11502-2 |