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SNP Microarray−Products of Conception (POC)/Tissue (Reveal®)

CPT

81229

Synonyms
  • Reveal® SNP Microarray−Products of Conception (POC)/Tissue

Test Details

Methodology

SNP microarray analysis is performed using the Cytoscan® HD Accel platform, which uses 743,130 SNP probes and 2,029,441 NPCN probes with a median spacing of 0.818 kb.

Result Turnaround Time

12 - 18 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Use

This test will detect chromosomal imbalance that could be associated with developmental delay/congenital anomalies. It provides possible detection of uniparental disomy of any chromosome, the percent and location of homozygosity, including the degree of identity by descent. It will also allow the detection of complete or partial molar pregnancies.

Special Instructions

Pertinent medical findings must accompany test request form. For formalin fixed paraffin embedded blocks or slides, test number will be updated to Microarray-Products of Conception (POC) Reveal® FFPE [511997]. If <8 mg nonfixed tissue is received, sample will be assessed to run on the Oncoscan Microarray platform, and test number adjusted as necessary and client notified.

If prior NIPT studies have been performed, include copy of the report.

Limitations

Unable to determine genotypes on some formalin-fixed tissue (blocks/slides), allowing only genomic dosage to be determined. This SNP assay does not detect balanced rearrangements, low-level mosaicism (<10%), marker chromosomes that only contain heterochromatin or tetraploidy.

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

Custom Additional Information

The microarray will detect DNA copy-number changes at a threshold >1 Mb for deletions, >2 Mb for duplications.

Specimen Requirements

Specimen

Nonfixed tissue, products of conception (POC) or placental villus biopsy

Volume

>8 mg tissue

Container

Submit biopsies in a sterile tube with sterile Ringer's lactate, Hanks' balanced salt solution or Labcorp transport medium. The transport tube (a sterile specimen container with transport medium) is available from Labcorp. Please call customer service at 800-345-4363. Specialized kits are now available for POC microarray testing. To order, please contact your local Labcorp representative (PeopleSoft No. 89063, Catalogue item ID No. 39025G). If no transport media is available, sterile isotonic saline can be used for transport. Do not use urine containers for shipping.

Collection Instructions

Aseptically obtain a small piece of tissue with subepidermal layers. Best results are obtained from placental villi, which remain viable much longer than fetal tissue. This is especially true in stillborn cases in which both cord blood and tissue samples are often not viable. If specimen is a product of conception, submit biopsies in a sterile tube with sterile Ringer's lactate, Hanks' balanced salt solution or Labcorp transport media. Gross contamination and necrotic tissue cannot be processed.

Storage Instructions

Specimens should be sent to the laboratory immediately. Maintain specimen at room temperature. Specimen may be refrigerated if there is a delay in shipment. Do not freeze.

Causes for Rejection

Upon receipt, suitability of specimen will be determined by the Cytogenetics laboratory.

References

Coppinger J, Alliman S, Lamb A, et al. Whole-genome microarray analysis in prenatal specimens identifies clinically significant chromosome alterations without increase in results of unclear significance compared to targeted microarray. Prenatal Diagn. 2009 Dec; 29(12):1156-1166.19795450

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
510110 POC/Tissue Microarray 96984-0 510112 Specimen Type 31208-2
510110 POC/Tissue Microarray 96984-0 510113 # of Genotyping Targets N/A
510110 POC/Tissue Microarray 96984-0 510114 Array Type 96984-0
510110 POC/Tissue Microarray 96984-0 510116 Diagnosis 48000-4
510110 POC/Tissue Microarray 96984-0 510117 Interpretation 62365-2
510110 POC/Tissue Microarray 96984-0 510118 Director Review: 48672-0
510110 POC/Tissue Microarray 96984-0 512123 PDF 11502-2
Order Code510110
Order Code NamePOC/Tissue Microarray
Order Loinc96984-0
Result Code510112
Result Code NameSpecimen Type
UofM
Result LOINC31208-2
Order Code510110
Order Code NamePOC/Tissue Microarray
Order Loinc96984-0
Result Code510113
Result Code Name# of Genotyping Targets
UofM
Result LOINCN/A
Order Code510110
Order Code NamePOC/Tissue Microarray
Order Loinc96984-0
Result Code510114
Result Code NameArray Type
UofM
Result LOINC96984-0
Order Code510110
Order Code NamePOC/Tissue Microarray
Order Loinc96984-0
Result Code510116
Result Code NameDiagnosis
UofM
Result LOINC48000-4
Order Code510110
Order Code NamePOC/Tissue Microarray
Order Loinc96984-0
Result Code510117
Result Code NameInterpretation
UofM
Result LOINC62365-2
Order Code510110
Order Code NamePOC/Tissue Microarray
Order Loinc96984-0
Result Code510118
Result Code NameDirector Review:
UofM
Result LOINC48672-0
Order Code510110
Order Code NamePOC/Tissue Microarray
Order Loinc96984-0
Result Code512123
Result Code NamePDF
UofM
Result LOINC11502-2