CPT: 81599
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  • Liver disease
  • NIS4™
  • Non-alcoholic fatty liver disease (NAFLD)
  • Non-alcoholic steatohepatitis (NASH)

Special Instructions

This assay currently is not available in New York state.

Expected Turnaround Time

5 - 7 days

Specimen Requirements


Serum and whole blood


4 mL whole blood and 4 mL serum

Minimum Volume

1 mL whole blood and 2 mL serum


Whole Blood: Lavender-top (EDTA) tube;

Serum: Gel-barrier serum tube or red-top tube.


**Please draw whole blood sample first.**

Whole Blood: Draw blood into lavender-top (EDTA) tube and ensure tube is completely filled. Gently invert tube six times to mix. Label tube with patient name, identification number, date and time of collection. The usual precautions in the collection of venipuncture should be observed. The sample must be free of clots. Samples with any hematocrit disorders can lead to erroneous results.

Serum: Separate serum from cells within 30 to 60 minutes after collection. Transfer specimen to two separate plastic transport tubes. Freeze and ship frozen.

Storage Instructions

Hb A1c: Room temperature whole blood;

CHI3L1, A2M and miR-34A: Frozen serum.

Stability Requirements




Whole Blood


Room temperature

14 days

2 hours


14 days

2 hours


14 days

1 week: -20 °C

180 days: -70°C

Freeze/thaw cycles

Stable x3

Stable x3

Patient Preparation

Patient must fast at least 12 hours before the blood draw.

Causes for Rejection

Gross hemolysis; unfrozen serum

Test Details


Labcorp’s NASHnext is a non-invasive blood test that reflects both NASH activity and fibrosis in a single score by combining the results of four individual NASH-associated biomarkers. This test is intended for use in assigning the risk level of NASH.


This test was validated and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.


NIS4 test produces a score from the results of the following assays:

HbA1c, Whole Blood, Roche;

a2-macroglobulin, serum, Roche;

miR34a, serum RT-qPCR;

CH13L1/YKl40 serum, immunoassay.


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
504960 NASHnext (NIS4) 93692-2 504964 NIS4 Test 93692-2

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