Our global life sciences company brings diagnostic testing & drug development together.

Pregnenolone, Pre- and Post-ACTH Stimulation

CPT: 84140(x2)
Print Share


  • Pregnenolone (Two Specimens)

Special Instructions

For pregnenolone without stimulation, order Pregnenolone, Mass Spectrometry (Endocrine Sciences) [140707].

Draw blood for baseline pregnenolone. Inject cosyntropin: 15 μg/kg in neonates; 125 μg in children younger than and including two-year-old children; and 250 μg in children older than two years and adults. IM or IV (If IV, dilute cosyntropin in 2 to 5 mL of sterile saline and inject over two minutes). Draw blood for stimulated pregnenolone 60 minutes after. See the online Endocrine Appendix: ACTH Stimulation for expected values for various steroids at baseline and after ACTH stimulation.

Expected Turnaround Time

7 - 9 days

Related Information

Related Documents

For more information, please view the literature below.

Adrenal Steroid Response to ACTH: Pediatrics

    Specimen Requirements


    Serum (preferred) or plasma, frozen


    1 mL (each specimen)

    Minimum Volume

    0.4 mL (Note: This volume does not allow for repeat testing.)


    Red-top tube, gel-barrier tube, lavender-top (EDTA) tube, or green-top (heparin) tube


    Draw two samples: one baseline and one stimulated 60 minutes. If a tube other than a gel-barrier is used, transfer separated serum or plasma to a plastic transport tube. Note: Label each tube with patient's name, collection time, and date. Submit specimens simultaneously on the same test request form. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

    Storage Instructions


    Stability Requirements




    1 day


    2 years

    Causes for Rejection

    Nonserum or non-EDTA/heparin plasma specimen

    Test Details


    This test may involve significant risk and should be performed only by qualified personnel who are familiar with the test and who have taken adequate precautions to protect the safety of the patient. Every effort has been made to ensure accuracy in these recommendations, but clinicians must use their judgment and refer to specific pharmaceutical resources to determine appropriate drug dosages for their patients.


    High-pressure liquid chromatography (HPLC)/tandem mass spectrometry

    LOINC® Map

    Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
    500773 Pregnenolone, ACTH Stimulation 500774 Pregnenolone, Baseline ng/dL 2837-3
    500773 Pregnenolone, ACTH Stimulation 500775 Pregnenolone, Stimulated ng/dL 46100-4

    For Providers

    Please login to order a test

    Order a Test

    © 2021 Laboratory Corporation of America® Holdings and Lexi-Comp Inc. All Rights Reserved.

    CPT Statement/Profile Statement

    The LOINC® codes are copyright © 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at Additional information regarding LOINC® codes can be found at, including the LOINC Manual, which can be downloaded at