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17-Hydroxypregnenolone, Pre- and Post-ACTH Stimulation

CPT: 84143(x2)
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Synonyms

  • 17-Hydroxypregnenolone (Two Specimens)
  • Pregnenolone, 17-Hydroxy (Two Specimens)

Special Instructions

Draw blood for baseline 17-OH pregnenolone. Inject cosyntropin 250 μg IM or IV (If IV, dilute cosyntropin in 2-5 mL of sterile saline and inject over two minutes). Draw blood for stimulated 17-OH pregnenolone 60 minutes after. See the online Endocrine Appendix: ACTH Stimulation for expected values for various steroids at baseline and after ACTH stimulation.


Expected Turnaround Time

7 - 12 days


Related Information


Related Documents

For more information, please view the literature below.

Adrenal Steroid Response to ACTH: Pediatrics


    Specimen Requirements


    Specimen

    Serum (preferred) or plasma, frozen


    Volume

    1 mL (each specimen)


    Minimum Volume

    0.4 mL (Note: This volume does not allow for repeat testing.)


    Container

    Red-top tube, gel-barrier tube, lavender-top (EDTA) tube, or green-top (heparin) tube


    Collection

    Transfer separated serum or plasma to a plastic transport tube. Note: Label each tube with patient's name, collection time, and date. Submit specimens simultaneously on the same test request form. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.


    Storage Instructions

    Freeze


    Stability Requirements

    Temperature

    Period

    Refrigerated

    1 day

    Frozen

    2 years


    Causes for Rejection

    Non-EDTA/heparin plasma; recently administered isotopes


    Test Details


    Use

    This test may involve significant risk and should be performed only by qualified personnel who are familiar with the test and who have taken adequate precautions to protect the safety of the patient involved. Every effort has been made to ensure accuracy in these recommendations, but clinicians must use their judgment and refer to specific pharmaceutical resources to determine appropriate drug dosages for their patients.


    Methodology

    High-pressure liquid chromatography (HPLC) tandem mass spectrometry


    LOINC® Map

    Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
    500770 17-OH Pregnenolone, ACTH Stim. 500771 17-OH Pregnenolone Baseline ng/dL 6765-2
    500770 17-OH Pregnenolone, ACTH Stim. 500772 17-OH Pregnenolone Stimulated ng/dL 13604-4

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    The LOINC® codes are copyright © 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf