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GeneSeq® Immuno: Targeted Variant Analysis

CPT

To be determined. Updates will be made when available.

Synonyms

Mutation-specific sequencing, family testing, known familial variant

Test Details

Methodology

Next-generation sequencing to identify genetic variants, including small nucleotide variants (SNVs), insertions, deletions and copy number variants (CNVs)

Result Turnaround Time

14 - 21 days; in some cases, additional time may be required for confirmatory or reflex tests

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Test Includes

This test includes all genes included in any GeneSeq® Immuno panel.

Use

This testing is for a known variant documented in the family and is available only for genes included in GeneSeq® Immuno panels.

Special Instructions

This test currently is not approved for use in New York state.

The specific gene and variant(s) to be analyzed must be indicated on the test requisition form. Failure to indicate the gene and variant will result in testing delays. Please include a copy of the previously tested family member's laboratory report for documentation.

Please call 800-345-4363 to speak with a laboratory genetic coordinator before submitting specimens for targeted variant analysis. If previous testing was performed at an outside laboratory, submitting a positive control sample is highly recommended.

To test fetal specimens, including cord blood, order GeneSeq® Immuno: Targeted Variant, Fetal Analysis [483083].

Test orders must include an attestation that the provider has the patient's informed consent for genetic testing.

Limitations

Technologies used do not detect germline mosaicism and do not rule out the presence of large chromosomal aberrations, including rearrangements and gene fusions, or variants in regions or genes not included in this test, or possible inter/intragenic interactions between variants or repeat expansions. Variant classification and/or interpretation may change over time if more information becomes available. False positive or false negative results may occur for reasons that include rare genetic variants, sex chromosome abnormalities, pseudogene interference, blood transfusions, bone marrow transplantation, somatic or tissue-specific mosaicism, mislabeled samples or erroneous representation of family relationships.

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

Specimen Requirements

Specimen

Whole blood or PurFlock buccal swab kit or Oragene Dx 500 saliva kit

Volume

4 mL whole blood or PurFlock buccal swab kit or Oragene Dx 500 saliva kit

Minimum Volume

3 mL whole blood or PurFlock buccal swab kit or Oragene Dx 500 saliva kit

Container

Lavender-top (EDTA) tube or yellow-top (ACD-A) tube or PurFlock buccal swab kit or Oragene Dx 500 saliva kit

Collection Instructions

Standard phlebotomy; follow PurFlock buccal swab kit or Oragene Dx 500 saliva kit collection instructions. Do not eat, drink, smoke or chew gum 30 minutes prior to collection.

Stability Requirements

TemperaturePeriod
Room temperatureWhole blood: 14 days; buccal swab: 60 days; saliva: 60 days
RefrigeratedWhole blood: 30 days; buccal swab: unstable; saliva: unstable

Storage Instructions

Maintain specimen at room temperature or refrigerate at 4°C. Do not freeze.

Causes for Rejection

Frozen or hemolyzed specimen; quantity not sufficient for analysis; improper container

References

Tangye SG, Al-Herz W, Bousfiha A, et al. Human Inborn Errors of Immunity: 2022 Update on the Classification from the International Union of Immunological Societies Expert Committee. J Clin Immunol. 2022 Oct;42(7):1473-1507. PubMed 35748970

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
484069 PIDD Targeted Variant 484070 Specimen Type Pending
484069 PIDD Targeted Variant 484072 Indication Pending
484069 PIDD Targeted Variant 484073 Result Pending
484069 PIDD Targeted Variant 484074 Interpretation Pending
484069 PIDD Targeted Variant 484075 Recommendations Pending
484069 PIDD Targeted Variant 484076 Clinical Information Pending
484069 PIDD Targeted Variant 484077 Comments Pending
484069 PIDD Targeted Variant 484078 Methods/Limitations Pending
484069 PIDD Targeted Variant 484079 References Pending
484069 PIDD Targeted Variant 484080 Director Review/Release Pending
484069 PIDD Targeted Variant 484081 IMAGE Pending
Order Code484069
Order Code NamePIDD Targeted Variant
Order Loinc
Result Code484070
Result Code NameSpecimen Type
UofM
Result LOINCPending
Order Code484069
Order Code NamePIDD Targeted Variant
Order Loinc
Result Code484072
Result Code NameIndication
UofM
Result LOINCPending
Order Code484069
Order Code NamePIDD Targeted Variant
Order Loinc
Result Code484073
Result Code NameResult
UofM
Result LOINCPending
Order Code484069
Order Code NamePIDD Targeted Variant
Order Loinc
Result Code484074
Result Code NameInterpretation
UofM
Result LOINCPending
Order Code484069
Order Code NamePIDD Targeted Variant
Order Loinc
Result Code484075
Result Code NameRecommendations
UofM
Result LOINCPending
Order Code484069
Order Code NamePIDD Targeted Variant
Order Loinc
Result Code484076
Result Code NameClinical Information
UofM
Result LOINCPending
Order Code484069
Order Code NamePIDD Targeted Variant
Order Loinc
Result Code484077
Result Code NameComments
UofM
Result LOINCPending
Order Code484069
Order Code NamePIDD Targeted Variant
Order Loinc
Result Code484078
Result Code NameMethods/Limitations
UofM
Result LOINCPending
Order Code484069
Order Code NamePIDD Targeted Variant
Order Loinc
Result Code484079
Result Code NameReferences
UofM
Result LOINCPending
Order Code484069
Order Code NamePIDD Targeted Variant
Order Loinc
Result Code484080
Result Code NameDirector Review/Release
UofM
Result LOINCPending
Order Code484069
Order Code NamePIDD Targeted Variant
Order Loinc
Result Code484081
Result Code NameIMAGE
UofM
Result LOINCPending