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Herpes Simplex Virus (HSV) Types 1 and 2, NAA

CPT: 87529(x2)
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Expected Turnaround Time

2 - 4 days



Specimen Requirements


Specimen

Vesicular fluid, ulcerated lesions, vaginal, or endocervical cells collected by one of the methods described below


Volume

One Aptima® swab or entire liquid cytology vial


Minimum Volume

One Aptima® swab or entire liquid cytology vial


Container

Aptima® unisex or Aptima® vaginal swab transport or ThinPrep® liquid cytology vial


Collection

Lesion/vesicle swab: Unroof or scrape the lesion with an Aptima® swab.

Vaginal swab: Care provider specimen: Collect vaginal fluid sample using the GenProbe® Aptima® vaginal swab kit by contacting the swab to the lower third of the vaginal wall and rotating the swab for 10 to 30 seconds to absorb fluid. Immediately place the swab into the transport tube and carefully break the swab shaft against the side of the tube. Tightly screw on the cap. Patient self-collection: Partially open the package of the GenProbe® Aptima® vaginal swab kit. Do not touch the soft tip or lay the swab down. If the soft tip is touched, the swab is laid down, or the swab is dropped, use a new Aptima® swab specimen collection kit. Remove the swab. Carefully insert the swab into the vagina about two inches past the vaginal opening and gently rotate the swab for 10 to 30 seconds, making sure the swab touches the walls of the vagina so that moisture is absorbed by the swab. Withdraw the swab without touching the skin. Immediately place the swab into the transport tube and carefully break the swab shaft against the side of the tube. Tightly screw on the cap.

Endocervical swab in Aptima®: Remove excess mucus from the cervical os and surrounding mucosa using the cleaning swab (white-shafted swab in the package with red printing). Discard this swab. Insert the specimen collection swab (blue-shafted swab in the package with green printing) into the endocervical canal. Gently rotate the swab clockwise for 10 to 30 seconds in the endocervical canal to ensure adequate sampling. Withdraw the swab carefully; avoid contact with the vaginal mucosa. Remove the cap from the swab specimen transport tube and immediately place the specimen collection swab into the transport tube. Carefully break the swab shaft at the scoreline using care to avoid splashing of the contents. Recap the swab specimen transport tube tightly.

ThinPrep® Vial − Broom or Brush/Spatula

Broom-like collection technique: Obtain a sample from the cervix using a broom-like device by inserting the brush portion into the cervical os and then rotating the brush five times. Rinse the collection device in the PreservCyt® solution by pushing the brush into the bottom of the vial 10 times, forcing the bristles to bend apart to release the cervical material. As a final step, twirl the brush between the thumb and forefinger vigorously to release additional cellular material. Discard the collection device. Tighten the cap on the ThinPrep® vial so that the torque line on the cap passes the torque line on the vial.

Brush/spatula technique: Insert the brush into the endocervical canal until only the bottommost fibers are exposed. Slowly rotate the brush ¼ to ½ turn in one direction. Do not over-rotate the brush. Then, rotate the brush in the PreservCyt® solution 10 times while pushing against the wall of the ThinPrep® vial. Swirl the brush vigorously to release additional material. Discard the brush. Obtain an adequate sample from the ectocervix using a plastic spatula. Swirl vigorously in the ThinPrep® vial 10 times and discard the spatula. Tighten the cap on the ThinPrep® container so that the torque line on the cap passes the torque line on the vial.


Storage Instructions

Maintain specimen at room temperature.


Causes for Rejection

Urine specimen; specimen transported under inappropriate conditions; bacterial swabs; unlabeled specimen or discrepancy between specimen label and test request form; Aptima® COMBO 2 (AC2) swab specimen transport tube with two swabs or swab not supplied by Gen-Probe® or no swab; ProbeTec™ swabs; female urethral swab; SurePath™ vial; Aptima® swab transports >7 days from collection


Test Details


Use

Detect and type active HSV shedding.


Limitations

This test is intended for use as an aid in the diagnosis of herpes simplex virus (HSV) infections with active viral shedding; it also differentiates HSV-1 from HSV-2. Negative HSV NAA results indicate lack of viral shedding but do not confirm absence of previous infection.


Methodology

Nucleic acid amplification (NAA)


LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
188056 HSV NAA 188057 HSV 1 NAA 16130-7
188056 HSV NAA 188058 HSV 2 NAA 16131-5

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CPT Statement/Profile Statement

The LOINC® codes are copyright © 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf