Specimen processing (i.e., N-acetyl-L-cystine-sodium hydroxide treatment or equivalent, concentration, grinding, both or neither), mycobacterial culture, and smear when appropriate (smears are not performed on blood or when there is less than 2 mL of fluid). Identification by DNA probes or sequencing will be performed at an additional charge. This culture will often detect Nocardia species and other aerobic actinomyces, and identification appropriate for these organisms will be included.
43 - 56 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
5 mL sputum
Sterile container with tight screw-cap seal
Determined by submitting institution
Refrigerate. If sample is to be split for other tests, specimen should be divided at the time of collection so that each portion is transported at the appropriate temperature.
Detect and identify Mycobacterium tuberculosis complex and an rpoB mutation that is associated with rifampin resistance
Nucleic Acid Amplification (NAA), nested real-time polymerase chain reaction (PCR); concentrated specimen with broth-based and/or agar-based culture. Culture is held for six weeks before negative is reported. Organisms are identified by use of DNA probes, MALDI-TOF and/or nucleic acid sequencing.
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