Mycobacterium tuberculosis Complex Detection and Rifampin Resistance (PCR), Acid-Fast Bacillus (AFB) Smear, and AFB Culture (Sputum)

CPT: 87116; 87206; 87556; 87798

Special Instructions

Specimen processing (i.e., N-acetyl-L-cystine-sodium hydroxide treatment or equivalent, concentration, grinding, both or neither), mycobacterial culture, and smear when appropriate (smears are not performed on blood or when there is less than 2 mL of fluid). Identification by DNA probes or sequencing will be performed at an additional charge. This culture will often detect Nocardia species and other aerobic actinomyces, and identification appropriate for these organisms will be included.


Expected Turnaround Time

43 - 56 days


Related Documents


Specimen Requirements


Volume

5 mL sputum


Minimum Volume

3 mL


Container

Sterile container with tight screw-cap seal


Collection

Determined by submitting institution


Storage Instructions

Refrigerate. If sample is to be split for other tests, specimen should be divided at the time of collection so that each portion is transported at the appropriate temperature.


Test Details


Use

Detect and identify Mycobacterium tuberculosis complex and an rpoB mutation that is associated with rifampin resistance


Methodology

Nucleic Acid Amplification (NAA), nested real-time polymerase chain reaction (PCR); concentrated specimen with broth-based and/or agar-based culture. Culture is held for six weeks before negative is reported. Organisms are identified by use of DNA probes, MALDI-TOF and/or nucleic acid sequencing.


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