Organism Identification by Matrix-assisted Laser Desorption/Ionization Time-of-Flight MS With Reflex to Sequence-based Identification

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Synonyms

  • MALDI-TOF Organism Identification

Special Instructions

Susceptibility testing may be requested for an additional charge. The client submitting the isolate for testing should provide Labcorp with clinical and testing information regarding test procedures already performed at the submitting laboratory in order to expedite testing on receipt.

Broth medium containing a pure culture may be submitted for testing; however, additional time will be required in order to perform subculture to solid media prior to initiation of MALDI testing. Testing will reflex to sequencing when ID cannot be determined by MALDI-TOF.


Expected Turnaround Time

5 - 15 days


Related Documents


Specimen Requirements


Specimen

Pure culture isolate of bacteria, yeast, filamentous fungi, AFB, Nocardia, or aerobic Actinomycetes


Volume

Pure culture (visible colonies should be present). Bacterial culture transport swab with media inoculated with the isolate from a pure culture or agar slant in a screw-cap container packed as an etiologic agent. Maintain a subculture at the submitting laboratory.


Minimum Volume

Viable colonies macroscopically visible on culture medium; one bacterial culture transport swab with media.


Container

Bacterial swab transport inoculated with the isolate from a pure culture or agar slant in screw-cap container packed as an etiologic agent. Maintain a subculture at the submitting laboratory.


Storage Instructions

Room temperature


Causes for Rejection

Isolate nonviable; unlabeled specimen or name discrepancy between the specimen and the name in the computer or on the test request form; isolate transported under inappropriate conditions; broken tube or plate; mixed culture; single specimen identifier


Test Details


Use

Determine the identification of isolates (ie, bacterial, mycobacterial, etc) from another laboratory that has performed culture of the clinical specimen. These isolates may include strict aerobes, facultative anaerobes, anaerobes, yeast, mycobacteria, Nocardia and/or Actinomycetes.


Limitations

Broth medium containing a pure culture may be submitted for testing; however, additional time will be required in order to perform subculture to solid media prior to initiation of MALDI testing.

The technology is limited to what is claimed in the spectral database. If organism is not obtained, the test will reflex to identification by ITS gene sequencing.


Methodology

Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry is the primary identification method. Ancillary testing (ie, 16S gene sequencing, ITS gene sequencing, conventional biochemicals) will be employed as required.


References

Dekker JP, Branda JA. MALDI-TOF mass spectrometry in the clinical microbiology laboratory. Clin Micro Newsletter. 2011 Jun 15; 33(12): 87-93.
Khot PD, Fisher MA. Mass spectrometry in the clinical microbiology laboratory, Part II: MALDI-TOF MS. Clin Micro Newsletter. 2012 Sep 15; 34(18): 143-147.
Lavigne J-P, Espinal P, Dunyach-Remy C, Messad N, Pantel A, Sotto A. Mass spectrometry: A revolution in clinical microbiology? Clin Chem Lab Med. 2013 Feb; 51(2):257-270. 23072853
Patel R. MALDI-TOF mass spectrometry: Transformative proteomics for clinical microbiology. Clin Chem. 2013 Feb; 59(2):340-342. 22665917
Verroken A, Janssens M, Berhin C, et al. Evaluation of matrix-assisted laser desorption ionization−time of flight mass spectrometry for identification of Nocardia species.J Clin Microbiol. 2010 Nov; 48(11):4015-4021. 20861335
El-Khéchine A, Couderc C, Flaudrops C, Raoult D, Drancourt M. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identification of mycobacteria in routine clinical practice. PLos One. 2011; 6(9):e24720. 21935444

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
183402 Organism ID by MALDI 76346-6 183403 Organism ID by MALDI 76346-6
Reflex Table for Organism ID by MALDI
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 183404 Aerobic ID by MALDI 183404 Aerobic ID by MALDI 75756-7
Reflex 2 183285 MALUID 183286 Organism ID by Sequencing 41852-5
Reflex 3 183269 Aerobic Bacteria, ID by Seq. 183270 Aerobic Bacteria, ID by Seq. 42803-7
Reflex 4 183271 Organism Identification 183272 Organism Identification 42803-7
Reflex Table for Organism ID by MALDI
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 183404 Aerobic ID by MALDI 183404 Aerobic ID by MALDI 75756-7
Reflex 2 183285 MALUID 183286 Organism ID by Sequencing 41852-5
Reflex 3 183301 Anaerobic Bacteria, ID by Seq. 183302 Anaerobic Bacteria, ID by Seq. 42803-7
Reflex 4 183303 Organism Identification 183304 Organism Identification 42803-7
Reflex Table for Organism ID by MALDI
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 183404 Aerobic ID by MALDI 183404 Aerobic ID by MALDI 75756-7
Reflex 2 183285 MALUID 183286 Organism ID by Sequencing 41852-5
Reflex 3 183277 Nocardia, ID by Sequencing 183278 Nocardia, ID by Sequencing 55096-2
Reflex 4 183279 Organism Identification 183280 Organism Identification 55096-2
Reflex Table for Organism ID by MALDI
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 183404 Aerobic ID by MALDI 183404 Aerobic ID by MALDI 75756-7
Reflex 2 183285 MALUID 183286 Organism ID by Sequencing 41852-5
Reflex 3 183293 Mycobacteria, ID by Seq. 183294 Mycobacteria, ID by Seq. 9825-1
Reflex 4 183295 Organism Identification 183296 Organism Identification 9825-1
Reflex Table for Organism ID by MALDI
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 183404 Aerobic ID by MALDI 183404 Aerobic ID by MALDI 75756-7
Reflex 2 183285 MALUID 183286 Organism ID by Sequencing 41852-5
Reflex 3 183319 Yeast, ID by Sequencing 183320 Yeast, ID by Sequencing 42804-5
Reflex 4 183321 Organism Identification 183322 Organism Identification 42804-5
Reflex Table for Organism ID by MALDI
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 183404 Aerobic ID by MALDI 183404 Aerobic ID by MALDI 75756-7
Reflex 2 183285 MALUID 183286 Organism ID by Sequencing 41852-5
Reflex 3 183327 Mold, ID by Sequencing 183328 Mold, ID by Sequencing 42804-5
Reflex 4 183329 Organism Identification 183330 Organism Identification 42804-5
Reflex Table for Organism ID by MALDI
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 183405 Anaerobic ID by MALDI 183405 Anaerobic ID by MALDI 75756-7
Reflex 2 183285 MALUID 183286 Organism ID by Sequencing 41852-5
Reflex 3 183269 Aerobic Bacteria, ID by Seq. 183270 Aerobic Bacteria, ID by Seq. 42803-7
Reflex 4 183271 Organism Identification 183272 Organism Identification 42803-7
Reflex Table for Organism ID by MALDI
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 183406 Nocardia ID by MALDI 183406 Nocardia ID by MALDI 76346-6
Reflex 2 183285 MALUID 183286 Organism ID by Sequencing 41852-5
Reflex 3 183269 Aerobic Bacteria, ID by Seq. 183270 Aerobic Bacteria, ID by Seq. 42803-7
Reflex 4 183271 Organism Identification 183272 Organism Identification 42803-7
Reflex Table for Organism ID by MALDI
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 183407 Mycobacteria ID by MALDI 183407 Mycobacteria ID by MALDI 76346-6
Reflex 2 183285 MALUID 183286 Organism ID by Sequencing 41852-5
Reflex 3 183269 Aerobic Bacteria, ID by Seq. 183270 Aerobic Bacteria, ID by Seq. 42803-7
Reflex 4 183271 Organism Identification 183272 Organism Identification 42803-7
Reflex Table for Organism ID by MALDI
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 183408 Yeast ID by MALDI 183408 Yeast ID by MALDI 76346-6
Reflex 2 183285 MALUID 183286 Organism ID by Sequencing 41852-5
Reflex 3 183269 Aerobic Bacteria, ID by Seq. 183270 Aerobic Bacteria, ID by Seq. 42803-7
Reflex 4 183271 Organism Identification 183272 Organism Identification 42803-7
Reflex Table for Organism ID by MALDI
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 183428 Mold ID by MALDI 183428 Mold ID by MALDI Pending
Reflex 2 183327 MALUID 183328 Mold, ID by Sequencing 42804-5
Reflex 3 183329 Organism Identification 183330 Organism Identification 42804-5
Reflex Table for Organism ID by MALDI
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 183327 Mold, ID by Sequencing 183328 Mold, ID by Sequencing 42804-5
Reflex 2 183329 SEQID2 183330 Organism Identification 42804-5

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