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CPT: 86352
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  • Cellular Function Assay (involving stimulation and detection of a biomarker)
  • Cylex Immune Assay
  • Immuno Test
  • Immunosuppression Level Assay

Special Instructions

Prior to initial submission of specimens for this test, contact HLA customer service (800-533-1037) to establish special specimen collection and transportation arrangements. These specimen collection and transportation arrangements must be observed in order to obtain acceptable results.

Expected Turnaround Time

2 - 4 days

Related Documents

For more information, please view the literature below.

ImmuKnow® Immune Cell Function Assay

Specimen Requirements


Whole blood (sodium heparin only)


6 to 8 mL

Minimum Volume

2 to 4 mL


Green-top (sodium heparin) tube only


Do not use lithium heparin, ACD, or EDTA tubes. Assay must be set up within 30 hours of specimen collection. Specimens received after 30 hours are not acceptable for testing.

Storage Instructions

Maintain specimen at room temperature (ie, 18°C to 28°C) only. Specimen stable 30 hours; >30 hours specimen not viable. Do not refrigerate or freeze specimen.

Causes for Rejection

Specimen chilled, refrigerated, or frozen; >30 hours since collection; clotted or hemolyzed specimen; lithium heparin used

Test Details


This test is a transplant physician's means of obtaining and monitoring information about immune status. ImmuKnow® measures the adenosine triphosphate (ATP) response of stimulated peripheral blood lymphocytes.


PHA stimulation ATP luciferase assay. ImmuKnow® is a trademark of Viracor-IBT Laboratories Inc, Lee's Summit, Mo.


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
176120 ImmuKnow (R) 53939-5 176101 ImmuKnow (R) ng/mL ATP 53939-5

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