Test Details
Methodology
Immunofluorescence
Use
This test is intended for confirmation of IgA antibody presence to aid in the diagnosis of pemphigoid, pemphigus, epidermolysis bullosa acquisita and bullous lupus erythematosus. IgA antibodies directed against the basement membrane zone (BMZ) are produced in pemphigoid. When serum contains anti-BMZ antibodies, the fluorescence pattern on sodium chloride (NaCI)-split skin substrate assists in differentiating pemphigoid from epidermolysis bullosa acquisita (EBA) and bullous lupus erythematosus (LE). Fluorescence on the roof (epidermal side) or on both epidermal and dermal sides of the split indicates pemphigoid, whereas fluorescence restricted to the dermal side is consistent with EBA or bullous LE. Additionally, patients with pemphigus produce IgA epidermal cell antibodies. The titer of epidermal cell surface (Intercellular) antibodies generally correlates with pemphigus disease activity.
Special Instructions
If reflex test is performed, additional charges/CPT code(s) may apply.
Limitations
This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.
Specimen Requirements
Specimen
Serum
Volume
1.0 mL
Minimum Volume
0.5 mL (Note: This volume does not allow for repeat testing.)
Container
Gel-barrier tube, red-top tube, transfer tube
Collection Instructions
If a tube other than a gel-barrier tube is used, transfer the separated serum to a plastic transport tube. Do not freeze the gel-barrier tube (pour off the serum first).
Stability Requirements
| Temperature | Period |
|---|---|
| Room temperature | 14 days |
| Refrigerated | 14 days |
| Frozen | 14 days |
| Freeze/thaw cycles | Stable x3 |
Storage Instructions
Room temperature
Causes for Rejection
Gross hemolysis; grossly lipemic; gross icterus
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 160098 | Skin IgA IFA | 160087 | Esophag cell surface IgA | Pending | ||
| 160098 | Skin IgA IFA | 160089 | Esophag BMZ IgA | Pending | ||
| 160098 | Skin IgA IFA | 160091 | Split Skin BMZ Roof IgA | Pending | ||
| 160098 | Skin IgA IFA | 160096 | Split Skin BMZ Floor IgA | Pending | ||
| Order Code | 160098 | |||||
| Order Code Name | Skin IgA IFA | |||||
| Order Loinc | ||||||
| Result Code | 160087 | |||||
| Result Code Name | Esophag cell surface IgA | |||||
| UofM | ||||||
| Result LOINC | Pending | |||||
| Order Code | 160098 | |||||
| Order Code Name | Skin IgA IFA | |||||
| Order Loinc | ||||||
| Result Code | 160089 | |||||
| Result Code Name | Esophag BMZ IgA | |||||
| UofM | ||||||
| Result LOINC | Pending | |||||
| Order Code | 160098 | |||||
| Order Code Name | Skin IgA IFA | |||||
| Order Loinc | ||||||
| Result Code | 160091 | |||||
| Result Code Name | Split Skin BMZ Roof IgA | |||||
| UofM | ||||||
| Result LOINC | Pending | |||||
| Order Code | 160098 | |||||
| Order Code Name | Skin IgA IFA | |||||
| Order Loinc | ||||||
| Result Code | 160096 | |||||
| Result Code Name | Split Skin BMZ Floor IgA | |||||
| UofM | ||||||
| Result LOINC | Pending |
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 160088 | Esophag cell surface IgA Titer | 160088 | Esophag cell surface IgA Titer | Pending | |
| Reflex 1 | ||||||
| Order Code | 160088 | |||||
| Order Name | Esophag cell surface IgA Titer | |||||
| Result Code | 160088 | |||||
| Result Name | Esophag cell surface IgA Titer | |||||
| UofM | ||||||
| Result LOINC | Pending | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 160090 | Esophag BMZ IgA Titer | 160090 | Esophag BMZ IgA Titer | Pending | |
| Reflex 1 | ||||||
| Order Code | 160090 | |||||
| Order Name | Esophag BMZ IgA Titer | |||||
| Result Code | 160090 | |||||
| Result Name | Esophag BMZ IgA Titer | |||||
| UofM | ||||||
| Result LOINC | Pending | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 160092 | Split Skin BMZ Roof IgA Titer | 160092 | Split Skin BMZ Roof IgA Titer | Pending | |
| Reflex 1 | ||||||
| Order Code | 160092 | |||||
| Order Name | Split Skin BMZ Roof IgA Titer | |||||
| Result Code | 160092 | |||||
| Result Name | Split Skin BMZ Roof IgA Titer | |||||
| UofM | ||||||
| Result LOINC | Pending | |||||
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
|---|---|---|---|---|---|---|
| Reflex 1 | 160097 | Split Skin BMZ Floor IgA Titer | 160097 | Split Skin BMZ Floor IgA Titer | Pending | |
| Reflex 1 | ||||||
| Order Code | 160097 | |||||
| Order Name | Split Skin BMZ Floor IgA Titer | |||||
| Result Code | 160097 | |||||
| Result Name | Split Skin BMZ Floor IgA Titer | |||||
| UofM | ||||||
| Result LOINC | Pending | |||||