Herpes Simplex Virus (HSV) Types 1/2, DNA PCR

CPT: 87529(x2)
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  • HSV Types 1 and 2, DNA PCR (Real Time)

Special Instructions

HSV PCR is a single test that both detects the presence of HSV DNA and determines which type is present in the positive samples. There is no mechanism, therefore, for testing for HSV-1 without simultaneously testing for HSV-2.

Expected Turnaround Time

2 - 4 days

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Specimen Requirements


Swab, whole blood, serum, or plasma


One swab; or 1 mL whole blood, serum, or plasma

Minimum Volume

One swab; or 0.5 mL whole blood, serum, or plasma


Universal transport medium (swab), red-top tube, gel-barrier tube, lavender-top (EDTA) tube, or yellow-top (ACD) tube


To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Storage Instructions

Whole blood is stable at room temperature or refrigerated for seven days; do not freeze. Swab samples are stable at room temperature, refrigerated, or frozen for seven days. Serum or plasma samples are stable at room temperature or refrigerated for seven days or frozen for 90 days.

Test Details


This test is intended for use as an aid in the diagnosis of herpes simplex virus (HSV) infections; it also differentiates HSV-1 from HSV-2. PCR testing of blood, serum, or plasma samples is clinically useful only in potential cases of disseminated HSV infection (neonates, immunosuppressed individuals) and not as an aid in the diagnosis of either mucosal or CNS disease.


Real-time polymerase chain reaction (PCR)


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
138651 HSV 1/2 PCR 87431-3 139332 HSV-1 DNA 16130-7
138651 HSV 1/2 PCR 87431-3 139333 HSV-2 DNA 16131-5

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