Lipid Panel With Apolipoprotein B (ApoB)

CPT: 80061; 82172
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Test Includes

Cholesterol, total; Apolipoprotein B (ApoB); high-density lipoprotein (HDL) cholesterol; low-density lipoprotein (LDL) cholesterol (calculation); non-high-density lipoprotein (non-HDL) cholesterol (calculation = total cholesterol minus HDLC); triglycerides


Expected Turnaround Time

1 - 3 days


Related Documents


Specimen Requirements


Specimen

Serum or plasma


Volume

1 mL


Minimum Volume

0.5 mL (Note: This volume does not allow for repeat testing.)


Container

Spun NMR LipoTube (preferred); serum from a plain red-top tube, plasma from a lavender-top (EDTA-no gel), or green-top (heparin-no gel) tube is acceptable.


Collection

Collect specimen in NMR LipoTube (black-and-yellow-top tube), which is the preferred container. Plain red-top, green-top (heparin-no gel), or lavender-top (EDTA-no gel) tubes are also acceptable. Serum or plasma drawn in gel-barrier collection tubes other than the NMR LipoTube should not be used.

The NMR LipoTube is the only acceptable gel-barrier tube. Gently invert tube 8 to 10 times to mix contents and allow specimen to clot for 30 minutes upright at room temperature prior to centrifugation (Plasma tubes should not clot). Centrifuge specimen within two hours of collection at 1800 to 2200xg for 10 to 15 minutes to separate serum/plasma from the red cells and to avoid red cell contamination during shipment.

Note: All specimens should be centrifuged by the client, prior to shipment to Labcorp, to ensure sample integrity. Immediately after centrifugation, pipette separated red-top serum or green-top/lavender-top plasma into a transport tube and label accordingly (serum, heparin plasma, EDTA plasma).


Storage Instructions

Refrigerate.


Stability Requirements

Temperature

Period

Room temperature

LipoTube Serum: 7 days; Plain Serum: 5 days; EDTA Plasma: 7 days; Sodium Heparin Plasma: 6 days

Refrigerated

All tubes: 14 days

Frozen

All tubes: 14 days (Note: Triglyceride values in frozen samples with high values >400 mg/dL may be decreased more than 10% when frozen.)

Freeze/thaw cycles

ApoB serum tubes: Stable x4; NMR LipoTubes: Stable x1; All other tubes: Stable x5


Patient Preparation

Patient fasting is not required; however, in conditions where triglyceride values provide a priori diagnostic information, such as screening for familial hypercholesterolemia or early onset heart disease, pancreatitis, or confirming hypertriglyceridemia, the patient should be counseled to fast 12 to 14 hours prior to blood draw.


Causes for Rejection

Unspun specimens; plasma/serum contaminated with red cells; citrated plasma (light blue-top tube); gross hemolysis; specimen received in gel-barrier collection tube other than the LipoTube


Test Details


Use

This "Extended Lipid Panel" quantifies the components of a typical lipid panel (TC, HDL-C and TG) along with ApoB by nuclear magnetic resonance (NMR) spectroscopy using the Vantera NMR Clinical Analyzer. Results from the Extended Lipid Panel Assay can be used by physicians to assist in CVD risk assessment. The principal protein component of LDL particles, ApoB, has been shown to be associated with CVD and is also an important CVD risk factor.


Limitations

If triglyceride level is >800 mg/dL, LDL cholesterol will not be calculated.


Methodology

Nuclear magnetic resonance (NMR)

Note: The NMR method for Triglycerides, unlike the routine chemical method, is not affected by endogenous glycerol, which might be found in very rare clinical conditions such as Hyperglycerolemia (glycerol kinase deficiency-GKD), an X-linked genetic disorder. Large quantitative differences in the chemical and NMR method results may be attributable to high blood glycerol.


LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
123544 Lipid Panel+ApoB 123477 Cholesterol, Total mg/dL 2093-3
123544 Lipid Panel+ApoB 123476 Triglycerides mg/dL 2571-8
123544 Lipid Panel+ApoB 123475 HDL-C mg/dL 2085-9
123544 Lipid Panel+ApoB 123478 Non-HDL Cholesterol mg/dL 43396-1
123544 Lipid Panel+ApoB 123479 LDL-C (NIH Calc) mg/dL 13457-7
123544 Lipid Panel+ApoB 123491 Apolipoprotein B mg/dL 1884-6

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