Cortisol, AM & PM

CPT: 82533(x2)
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Special Instructions

Submit two specimens on the same test request form (one collected in the AM and one in the PM as directed by the ordering physician). Print collection times and dates for both specimens on the individual tubes and in clinical information.

This test may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R). It is recommended to ask all patients who may be indicated for this test about biotin supplementation. Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.

Expected Turnaround Time

1 - 2 days

Related Information

Related Documents

Specimen Requirements




1 mL AM specimen and 1 mL PM specimen

Minimum Volume

0.7 mL each (Note: This volume does not allow for repeat testing.)


Gel-barrier tube (preferred) or red-top tube


If a red-top tube is used, transfer separated serum to a plastic transport tube.

Storage Instructions

Room temperature

Stability Requirements



Room temperature

14 days


14 days


14 days

Freeze/thaw cycles

Stable x3

Causes for Rejection

Citrate plasma specimen; improper labeling

Test Details


Electrochemiluminescence immunoassay (ECLIA)

Reference Interval

AM: 6.2−19.4 μg/dL; PM: 2.3−11.9 μg/dL


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
104000 Cortisol, AM/PM 104018 Cortisol - AM ug/dL 9813-7
104000 Cortisol, AM/PM 104026 Cortisol - PM ug/dL 9812-9

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