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Cortisol, AM & PM

CPT: 82533(x2)
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Special Instructions

Submit two specimens on the same test request form (one collected in the AM and one in the PM as directed by the ordering physician). Print collection times and dates for both specimens on the individual tubes and in clinical information.

This test may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R). It is recommended to ask all patients who may be indicated for this test about biotin supplementation. Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.


Expected Turnaround Time

1 - 2 days


Related Information


Related Documents


Specimen Requirements


Specimen

Serum


Volume

0.8 mL AM specimen and 0.8 mL PM specimen


Minimum Volume

0.3 mL each (Note: This volume does not allow for repeat testing.)


Container

Red-top tube or gel-barrier tube


Collection

If a red-top tube is used, transfer separated serum to a plastic transport tube.


Storage Instructions

Room temperature.


Stability Requirements

Temperature

Period

Room temperature

14 days

Refrigerated

14 days

Frozen

14 days

Freeze/thaw cycles

Stable x3


Causes for Rejection

Citrate plasma specimen; improper labeling


Test Details


Methodology

Electrochemiluminescence immunoassay (ECLIA)


Reference Interval

AM: 6.2−19.4 μg/dL; PM: 2.3−11.9 μg/dL


LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
104000 Cortisol, AM/PM 104018 Cortisol - AM ug/dL 9813-7
104000 Cortisol, AM/PM 104026 Cortisol - PM ug/dL 9812-9

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