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Anti-dsDNA (Double-stranded) Antibodies

CPT: 86225
Updated on 08/17/2021
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Synonyms

  • Anti-N-DNA
  • Antinative DNA

Test Includes

Semiquantitative result of IgG class antibodies


Special Instructions

This test may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R). It is recommended to ask all patients who may be indicated for this test about biotin supplementation. Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.


Expected Turnaround Time

2 - 3 days

1 - 3 days

2 - 3 days


Related Documents


Specimen Requirements


Specimen

Serum


Volume

1 mL


Minimum Volume

0.5 mL (Note: This volume does not allow for repeat testing.)


Container

Red-top tube or gel-barrier tube


Storage Instructions

Room temperature


Stability Requirements

Temperature

Period

Room temperature

14 days

Refrigerated

14 days

Frozen

14 days

Freeze/thaw cycles

Stable x3


Causes for Rejection

Hemolysis; icterus; lipemia; bacterial contamination


Test Details


Use

Specific assay for confirming the diagnosis of systemic lupus erythematosus (SLE)


Limitations

Low antibody levels may be found in other connective tissue diseases.


Methodology

Multiplex flow immunoassay


Reference Interval

• Negative: <5 IU/mL

• Equivocal: 5−9 IU/mL

• Positive: >9 IU/mL


Additional Information

Antibodies to DNA, either single- or double-stranded, are found primarily in systemic lupus erythematosus, and are important, but not necessary or sufficient for diagnosing that condition. Such antibodies are present in 80% to 90% of SLE cases. They are also present in smaller fractions of patients with other rheumatic disorders, and in chronic active hepatitis, infectious mononucleosis, and biliary cirrhosis.

In the past, it was considered unnecessary to test for anti-DNA in patients with a negative test for antinuclear antibodies. A group of “ANA-negative lupus” patients has been described with anti-ssDNA and anti-SS-A/Ro and anti-SS-B/La; however, HEp-2 substrate is much more sensitive than frozen section substrates, and it is uncommon for anti-SS-A/Ro to be negative with these newer substrates.

This standard dsDNA detects both low- and high-affinity antibodies, providing a very sensitive test for diagnostic purposes; however, it is less predictive for severe nephritis, which is associated with the presence of high-affinity antibodies.

Following levels of anti-DNA antibody may be of use in evaluating response to therapy, but should be regarded as a guide rather than a rigid dictator of treatment. Antibody levels correlate particularly well with activity of lupus nephritis.

Procainamide and hydralazine may induce anti-DNA antibodies and antihistone antibodies.


LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
096339 Anti-dsDNA Antibodies 5130-0 096343 Anti-DNA (DS) Ab Qn IU/mL 5130-0

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