Testosterone, Free, Direct With Total Testosterone, LC/MS-MS

CPT: 84402; 84403
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Special Instructions

State patient's age and sex on the test request form.

Expected Turnaround Time

4 - 6 days

Specimen Requirements




2 mL

Minimum Volume

1.3 mL (Note: This volume does not allow for repeat testing.)


Red-top tube or gel-barrier tube


Serum should be transferred from cells within one hour of collection and transferred to a plastic transport tube.

Storage Instructions

Room temperature

Stability Requirements



Room temperature

7 days


7 days


14 days

Freeze/thaw cycles

Stable x3

Causes for Rejection

Plasma specimen; gross lipemia (may yield erroneously high testosterone results); improper labeling

Test Details


Evaluate hirsutism and masculinization in women; evaluate testicular function in clinical states in which testosterone-binding proteins may be altered (obesity, cirrhosis, thyroid disorders)


This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.


Free: Direct analog enzyme immunoassay (EIA); Total: liquid chromatography/tandem mass spectrometry (LC/MS-MS)


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
070195 Testosterone, Free+Total LC/MS 58952-3 070036 Testosterone, Total, LC/MS ng/dL 2986-8
070195 Testosterone, Free+Total LC/MS 58952-3 144981 Free Testosterone(Direct) pg/mL 2991-8

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