Nicotine and Metabolite, Quantitative, Urine

CPT: 80323
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  • Tobacco

Expected Turnaround Time

4 - 8 days

Related Documents

Specimen Requirements




1 mL

Minimum Volume

0.5 mL


Urine container, no preservative

Storage Instructions

Room temperature

Stability Requirements



Room temperature

14 days


14 days


14 days

Test Details


Measurement of nicotine and its metabolites to monitor success of smoking cessation programs, detect passive exposure, and evaluate nontobacco nicotine exposure


This test was developed and its performance characteristics determined by LabCorp. It has not been cleared or approved by the Food and Drug Administration.


Liquid chromatography/tandem mass spectrometry (LC/MS-MS)


Analytical Validation Report. Quantification of Nicotine and Metabolites by LC-MS/MS Analysis. Unpublished LabCorp Internal Document Number VR-NOQS-BN-2008-06.
James G, et al. A Highly Sensitive Method for the Quantitation of Nicotine, Cotinine, and Trans-3'-Hydroxycotinine in Human Plasma (EDTA). ASMS Poster 1014, 2007.
Moyer TP, Charlson JR, Enger RJ, et al. Simultaneous analysis of nicotine, nicotine metabolites, and tobacco alkaloids in serum or urine by tandem mass spectrometry, with clinically relevant metabolic profiles. Clin Chem. 2002 Sep; 48(9):1460-1471. 12194923
Xu X, Iba MM, Weisel CP. Simultaneous and sensitive measurement of anabasine, nicotine, and nicotine metabolites in human urine by liquid chromatography-tandem mass spectrometry. Clin Chem. 2004 Dec; 50(12):2323-2330. 15472033


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
070045 Nicotine and Metabolite, Ur Qn 3854-7 070108 Nicotine ng/mL 3854-7
070045 Nicotine and Metabolite, Ur Qn 3854-7 070107 Cotinine ng/mL 10366-3

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