Antiextractable Nuclear Antigens

CPT: 86235(x2)
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  • Anti-ENA
  • Anti-RNP
  • Anti-Sm

Test Includes

Semiquantitative result of IgG class antibodies

Expected Turnaround Time

1 - 2 days

Related Documents

Specimen Requirements




1 mL

Minimum Volume

0.5 mL (Note: This volume does not allow for repeat testing.)


Red-top tube or gel-barrier tube

Storage Instructions

Room temperature

Stability Requirements



Room temperature

14 days


14 days


14 days

Freeze/thaw cycles

Stable x3

Causes for Rejection

Hemolysis; icterus; lipemia; bacterial contamination

Test Details


Detect antibodies associated with SLE and mixed connective tissue disease. Anti-Sm is highly specific for SLE. Anti-RNP is found with a variety of rheumatoid diseases with high titers associated mainly with MCTD.


Multiplex flow immunoassay

Reference Interval

0.0−0.9 AI

Additional Information

Antibodies that produce a speckled ANA pattern are usually directed against extractable nucleoprotein complexes found in the nucleus and/or cytoplasm of substrate cells. Included in this group of antigens are RNP, Sm, SS-A, SS-B, Scl-70, and Jo-1.


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
006338 Antiextractable Nuclear Ag 016354 RNP Antibodies AI 29374-6
006338 Antiextractable Nuclear Ag 016362 Smith Antibodies AI 11090-8

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