Our global life sciences company brings diagnostic testing & drug development together.

Coombs', Direct

CPT: 86880
Print Share


  • DAT
  • Direct Antihuman Globulin Test
  • Direct Coombs'

Test Includes

Direct antiglobulin test with polyspecific antihuman globulin serum. Red cells found to be positive are further tested with monospecific antisera (anti-IgG and anticomplement).

Expected Turnaround Time

1 - 2 days

Related Documents

Specimen Requirements


Whole blood


4 mL

Minimum Volume

0.5 mL


Lavender-top (EDTA) tube or yellow-top (ACD) tube

Storage Instructions

Specimens should be tested as soon as possible or within 72 hours of collection. Samples that cannot be tested immediately should be stored at 2°C to 8°C. Do not freeze.

Causes for Rejection

Gross hemolysis; bacterial or other contamination; specimen more than five days old

Test Details


Detect in vivo coating of IgG and/or complement components on RBCs


The presence of potent cold autoagglutinins may interfere with test interpretation. Testing performed using clotted blood specimens may yield false-positive results with anticomplement.


Antiglobulin test

Reference Interval


Additional Information

Positive direct antiglobulin tests are associated with a variety of conditions which include hemolytic transfusion reaction, autoimmune hemolytic anemia, hemolytic disease of the fetus/newborn, and may be secondary to certain pathological conditions or drug therapy. Note: The presence of a positive direct antiglobulin test does not necessarily correlate with a pathological condition.


Fung MK, Grossman JB, Hillyer CD, Westoff CM, eds. AABB Technical Manual. 18th ed. Bethesda, Md: American Association of Blood Banks;2014.


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
006270 Coombs', Direct 1007-4 006270 Coombs', Direct 1007-4
Reflex Table for Coombs', Direct
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 006271 Anti-Complement+Anti-IgG 006272 Anti-IgG 1006-6
Reflex Table for Coombs', Direct
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 006271 Anti-Complement+Anti-IgG 006273 Anti-Complement 13946-9

For Providers

Please login to order a test

Order a Test

© 2021 Laboratory Corporation of America® Holdings and Lexi-Comp Inc. All Rights Reserved.

CPT Statement/Profile Statement

The LOINC® codes are copyright © 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at Additional information regarding LOINC® codes can be found at, including the LOINC Manual, which can be downloaded at