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Coombs', Direct

CPT: 86880
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Synonyms

  • DAT
  • Direct Antihuman Globulin Test
  • Direct Coombs'

Test Includes

Direct antiglobulin test with polyspecific antihuman globulin serum. Red cells found to be positive are further tested with monospecific antisera (anti-IgG and anticomplement).


Expected Turnaround Time

1 - 2 days


Related Documents


Specimen Requirements


Specimen

Whole blood


Volume

4 mL


Minimum Volume

0.5 mL


Container

Lavender-top (EDTA) tube or yellow-top (ACD) tube


Storage Instructions

Specimens should be tested as soon as possible or within 72 hours of collection. Samples that cannot be tested immediately should be stored at 2°C to 8°C. Do not freeze.


Causes for Rejection

Gross hemolysis; bacterial or other contamination; specimen more than five days old


Test Details


Use

Detect in vivo coating of IgG and/or complement components on RBCs


Limitations

The presence of potent cold autoagglutinins may interfere with test interpretation. Testing performed using clotted blood specimens may yield false-positive results with anticomplement.


Methodology

Antiglobulin test


Reference Interval

Negative


Additional Information

Positive direct antiglobulin tests are associated with a variety of conditions which include hemolytic transfusion reaction, autoimmune hemolytic anemia, hemolytic disease of the fetus/newborn, and may be secondary to certain pathological conditions or drug therapy. Note: The presence of a positive direct antiglobulin test does not necessarily correlate with a pathological condition.


References

Fung MK, Grossman JB, Hillyer CD, Westoff CM, eds. AABB Technical Manual. 18th ed. Bethesda, Md: American Association of Blood Banks;2014.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
006270 Coombs', Direct 1007-4 006270 Coombs', Direct 1007-4
Reflex Table for Coombs', Direct
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 006271 Anti-Complement+Anti-IgG 006272 Anti-IgG 1006-6
Reflex Table for Coombs', Direct
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 006271 Anti-Complement+Anti-IgG 006273 Anti-Complement 13946-9

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