Protein Electrophoresis, Random Urine With Reflex to IFE, Random Urine

CPT: 84156; 84166
Print Share

Synonyms

  • Electrophoresis, Protein, Random Urine With Reflex to IFE, Urine

Test Includes

Random urine protein electrophoresis with automatic reflex to urine immunofixation (IFE) at additional charge if M-spike is present.


Special Instructions

For 24-hour urine specimens, order test 123018.


Expected Turnaround Time

3 - 5 days


Related Documents


Specimen Requirements


Specimen

Urine (random)


Volume

20-mL aliquot


Minimum Volume

11-mL aliquot (Note: This volume does not allow for repeat testing.)


Container

12-mL plastic transport tube (Labcorp No. 23597) without preservative


Collection

Collect first morning urine; mix well.


Storage Instructions

Refrigerated (preferred) or room temperature


Stability Requirements

Temperature

Period

Room temperature

14 days

Refrigerated

14 days

Frozen

14 days

Freeze/thaw cycles

Stable x1


Causes for Rejection

Preservative added


Test Details


Use

Work up myeloma, macroglobulinemia of Waldenström, lymphoma, amyloidosis; differentiate between normal renal function, glomerular proteinuria, and tubular proteinuria. Increased glomerular permeability leads to higher concentrations of large proteins in the glomerular filtrate. Diminished tubular reabsorptive capacity results in a marked increase in urinary excretion of low molecular weight problems.


Limitations

May not detect pathologic light chains due to insufficient sensitivity. Should be followed with immunoelectrophoretic or immunofixation study performed on concentrated urine. Optimal specimen when looking for a free monoclonal light chain (Bence Jones protein) is a 24-hour collection.


Methodology

Electrophoresis on agarose media


Additional Information

A serum protein electrophoresis should be reviewed concurrently if one has not been recently studied. In nonselective glomerular proteinuria, the urine electrophoretic pattern is often a nonspecific one which may be called “mirror image” to that of the serum. Contamination of the urine with blood can give a similar pattern. With selective glomerular permeability, albumin, α1 proteins, and transferrin are the predominant proteins identified on the urine protein electrophoresis, with a relative absence of heavier molecular weight proteins (ie, α2-macroglobulin and immunoglobulins). With tubular proteinuria, low molecular weight proteins (α2-macroglobulin) are predominant, with trace amounts of albumin. So called “overflow proteinuria” occurs when low molecular weight proteins are filtered through the glomerulus in increased amounts.


LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
003715 PE(Rfx IFE), Random Ur 013664 Protein,Total,Urine mg/dL 2888-6
003715 PE(Rfx IFE), Random Ur 003718 Albumin, U % 13992-3
003715 PE(Rfx IFE), Random Ur 003719 Alpha-1-Globulin, U % 13990-7
003715 PE(Rfx IFE), Random Ur 003720 Alpha-2-Globulin, U % 13993-1
003715 PE(Rfx IFE), Random Ur 003721 Beta Globulin, U % 13994-9
003715 PE(Rfx IFE), Random Ur 003722 Gamma Globulin, U % 13995-6
003715 PE(Rfx IFE), Random Ur 003723 M-Spike, % % 42483-8
003715 PE(Rfx IFE), Random Ur 133800 Please note: 77202-0
003715 PE(Rfx IFE), Random Ur 003716 . N/A
003715 PE(Rfx IFE), Random Ur 003724 PDF 11502-2
Reflex Table for .
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 120725 IFE Reflex, Random Urine 013482 Immunofixation Result, Urine 13440-3

For Providers

Please login to order a test

Order a Test

© 2021 Laboratory Corporation of America® Holdings and Lexi-Comp Inc. All Rights Reserved.

CPT Statement/Profile Statement

The LOINC® codes are copyright © 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf