Lyme Disease, Western Blot

CPT: 86617(x2)
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Test Details

Synonyms

  • Borrelia burgdorferi Antibodies

Test Includes

Western blot analysis and interpretations for IgG- and for IgM-specific antibodies

Use

Detect antibodies specific for B burgdorferi. This test is intended as the second step in testing serum samples found to have been equivocal or positive using an ELISA or IFA test as the first step to provide supportive evidence of infection with B burgdorferi.

Methodology

Western blot

Reference Interval

Negative

Additional Information

Provides both IgM and IgG Western blot results. Increased specificity reduces cross-reactivity from other spirochetes. Detect and analyze antibodies against specific B burgdorferi proteins. The sensitivity of a Western blot assay by itself is not sufficient to assess Lyme infection adequately. Both EIA and Western blot tests should be performed.

Specimen Requirements

Specimen

Serum

Volume

1 mL

Minimum Volume

0.5 mL

Container

Red-top tube or gel-barrier tube

Storage Instructions

Maintain specimen at room temperature.

Causes for Rejection

Hemolysis; lipemia; gross bacterial contamination

Clinical Information

Special Instructions

This test is intended as the second step in testing serum samples found to have been equivocal or positive using an ELISA or IFA test as the first step to provide supportive evidence of infection with B burgdorferi. For CSF, order test 160457.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
163600 Lyme, Western Blot, Serum 13503-8 163635 IgG P93 Ab. 9597-6
163600 Lyme, Western Blot, Serum 13503-8 163605 IgG P66 Ab. 9596-8
163600 Lyme, Western Blot, Serum 13503-8 163623 IgG P58 Ab. 9595-0
163600 Lyme, Western Blot, Serum 13503-8 163641 IgG P45 Ab. 9594-3
163600 Lyme, Western Blot, Serum 13503-8 163607 IgG P41 Ab. 9593-5
163600 Lyme, Western Blot, Serum 13503-8 163598 IgG P39 Ab. 9592-7
163600 Lyme, Western Blot, Serum 13503-8 163633 IgG P30 Ab. 9591-9
163600 Lyme, Western Blot, Serum 13503-8 163636 IgG P28 Ab. 9590-1
163600 Lyme, Western Blot, Serum 13503-8 163642 IgG P23 Ab. 9589-3
163600 Lyme, Western Blot, Serum 13503-8 163627 IgG P18 Ab. 9588-5
163600 Lyme, Western Blot, Serum 13503-8 163640 Lyme IgG WB Interp. 13502-0
163600 Lyme, Western Blot, Serum 13503-8 163616 IgM P41 Ab. 9587-7
163600 Lyme, Western Blot, Serum 13503-8 163599 IgM P39 Ab. 9599-2
163600 Lyme, Western Blot, Serum 13503-8 163638 IgM P23 Ab. 9598-4
163600 Lyme, Western Blot, Serum 13503-8 163639 Lyme IgM WB Interp. 13503-8

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CPT Statement/Profile Statement

The LOINC® codes are copyright © 1994-2018, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf