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Herpes Simplex Virus (HSV) Culture Without Typing
- Nontyping Culture
- Viral Culture, Herpes Simplex
Enzyme-linked virus-inducible system (ELVIS®). In this cell line, HSV infection causes the expression of a β-galactosidase “reporter gene,” and it is the expression of this gene that can be used to monitor infection by HSV.
Infectious HSV is rarely present in CSF during encephalitis, resulting in a poor recovery by culture (<5% in adults, <50% in children). Studies have shown a >98% detection of HSV DNA in CSF by polymerase chain reaction (PCR), and this method is now considered the standard for diagnosis of HSV encephalitis. For HSV PCR testing, please request test 138651. HSV is occasionally isolated from the CSF of patients with HSV 2 meningitis and of neonates with congenital herpes, and from urine of patients with primary genital HSV infections concurrent with cystitis.
Vesicular fluid, ulcerated lesions, pharyngeal and throat swabs, urine, cerebrospinal fluid (CSF), autopsy and biopsy material, eye exudates, and vaginal swabs
Swab in viral transport medium, 1 mL fluid, 0.5 g tissue in transport medium
Specimen is best collected within the first three days following the appearance of a lesion but no more than seven days. After collection, remove the cap from the transport medium tube, and insert the swab aseptically into the transport tube and break the swab shaft. Replace the cap so that the swab will not interfere with closure and allow the tube to leak. Label the tube with patient identification and transport specimen to the laboratory at 4°C.
Maintain specimen at 4°C.
Causes for Rejection
Bacterial swab specimen; specimen received in grossly leaking transport container; dry specimen; specimen submitted in fixative or additive; specimen received in expired transport media or incorrect transport devices; inappropriate specimen transport conditions; specimen received after prolonged delay in transport (usually more than 72 hours); specimen stored or transported at room temperature; wooden shaft swab in transport device; calcium alginate swab; unlabeled specimen or name discrepancy between specimen and request label
Submit one specimen per test requested. Specify the exact specimen source/origin (eg, genital lesion). Indicate a specific test number on the request form. Check expiration date of transport; do not use expired devices.
|Order Code||Order Code Name||Order Loinc||Result Code||Result Code Name||UofM||Result LOINC|
|186072||HSV Culture Without Typing||5859-4||186073||HSV Culture Without Typing||5859-4|