Fluorescence in situ Hybridization (FISH), Paraffin Block

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Test Details

Synonyms

  • Cytogenetics Fluorescence in situ Hybridization on Paraffin-embedded Tissue

Use

For specific FISH probe analysis of tissue specimens

Limitations

Samples in formaldehyde greater than 12 hours may reduce hybridization efficiency.

This test was developed, and its performance characteristics determined, by LabCorp. It has not been cleared or approved by the US Food and Drug Administration (FDA). The FDA has determined that such clearance or approval is not necessary.

Methodology

Fluorescence in situ hybridization (FISH) from formalin-fixed tissue-embedded paraffin (4-micron to 5-micron sections); H&E for pathology review.

Specimen Requirements

Specimen

Formalin/bone marrow-fixed (not decalcified); paraffin-embedded tissue or six 4-micron to 5-micron thick section slides

Volume

One paraffin block or six 4-micron to 5-micron thick section slides

Minimum Volume

Partial paraffin block to make several slides/probe

Container

Paraffin block transport pouch

Collection

Specimen should be fixed in 10% neutral-buffered formalin or formaldehyde.

Storage Instructions

Maintain specimen at room temperature.

Causes for Rejection

Improperly embedded prep; poor fixation <6 hours and >72 hours; decalcified bone or bone core; broken or dirty slides; absence of tumor or quantity not sufficient for analysis

Clinical Information

Special Instructions

This test number is used for tracking FISH paraffin samples to the testing facility. Billing will occur after testing is completed.

Pertinent medical findings and probe desired must accompany request for FISH. If prior chromosome analysis has been performed, include a copy of the report. Please direct questions to genetics customer service at 800-345-4363.

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The LOINC® codes are copyright © 1994-2018, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf