Also known as:Insulin C-peptide; Connecting Peptide Insulin; Proinsulin C-peptide
Related tests:Insulin; Glucose
Why Get Tested?
To help evaluate insulin production by the beta cells in the pancreas or to help determine the cause of low blood glucose (hypoglycemia)
When to Get Tested?
When you have diabetes and your health practitioner wants to determine if you are producing enough of your own insulin or if it is time to supplement oral medication with insulin injections or an insulin pump; when your health practitioner suspects that you have insulin resistance; when you have documented hypoglycemia
A blood sample drawn from a vein in your arm and sometimes a 24-hour urine sample
Test Preparation Needed?
Fasting for 8 to 10 hours before blood testing is usually required.
How is it used?
C-peptide testing can be used for a few different purposes. C-peptide is a substance produced by the beta cells in the pancreas when proinsulin splits apart and forms one molecule of C-peptide and one molecule of insulin. Insulin is the hormone that is vital for the body to use its main energy source, glucose. Since C-peptide and insulin are produced at the same rate, C-peptide is a useful marker of insulin production.
The following are some purposes of C-peptide testing:
- A C-peptide test is not ordered to help diagnose diabetes, but when a person has been newly diagnosed with diabetes, it may be ordered by itself or along with an insulin level to help determine how much insulin a person's pancreas is still producing (endogenous insulin).
- In type 2 diabetes, the body is resistant to the effects of insulin (insulin resistance) and it compensates by producing and releasing more insulin, which can also lead to beta cell damage. Type 2 diabetics usually are treated with oral drugs to stimulate their body to make more insulin and/or to cause their cells to be more sensitive to the insulin that is already being made. Eventually, because of the beta cell damage, type 2 diabetics may make very little insulin and require injections. Any insulin that the body does make will be reflected in the C-peptide level; therefore, the C-peptide test can be used to monitor beta cell activity and capability over time and to help a health practitioner determine when to begin insulin treatment.
- People who are on insulin therapy, regardless of the source of the insulin, may develop antibodies to insulin. These typically interfere with tests for insulin, making it nearly impossible to directly evaluate endogenous insulin production. In these cases, C-peptide measurement is a useful alternative to testing for insulin.
- C-peptide measurements can also be used in conjunction with insulin and glucose levels to help diagnose the cause of documented hypoglycemia and to monitor its treatment. Symptoms of hypoglycemia may be caused by excessive supplementation of insulin, alcohol consumption, inherited liver enzyme deficiencies, liver or kidney disease, or by insulinomas.
- The C-peptide test may be used to help diagnose Insulinomas. These are tumors of the islet cells in the pancreas that can produce uncontrolled amounts of insulin and C-peptide and can cause acute episodes of hypoglycemia. C-peptide tests may be used to monitor the effectiveness of insulinoma treatment and to detect recurrence.
- Sometimes a C-peptide test may be used to help evaluate a person diagnosed with metabolic syndrome, a set of risk factors that includes abdominal obesity, increased blood glucose and/or insulin resistance, unhealthy blood lipid levels, and high blood pressure (hypertension).
- Rarely, when someone has had his pancreas removed or has had pancreas islet cell transplants, intended to restore the ability to make insulin, C-peptide levels may be used to verify the effectiveness of treatment and continued success of the procedure.
When is it ordered?
C-peptide levels may be ordered when a person has been newly diagnosed with type 1 diabetes as part of an evaluation of the person's "residual beta cell function."
With type 2 diabetes, the test may be ordered on a regular basis when a health practitioner wants to monitor the status of a person's beta cells and insulin production over time and to determine if/when insulin injections may be required.
C-peptide levels may be done when there is documented acute or recurring low blood glucose (hypoglycemia) and/or excess insulin is suspected. Symptoms of hypoglycemia include:
- Blurred vision
- In severe cases, seizures and loss of consciousness
However, many of these symptoms can occur with other conditions as well.
When a person has been diagnosed with an insulinoma, a C-peptide test may be ordered periodically to monitor the effectiveness of treatment and to detect tumor recurrence.
Rarely, C-peptide levels may be monitored over time when someone has had his pancreas removed or has had pancreas islet cell transplants.
What does the test result mean?
A high level of C-peptide generally indicates a high level of endogenous insulin production. This may be in response to a high blood glucose caused by glucose intake and/or insulin resistance. A high level of C-peptide is also seen with insulinomas and may be seen with low blood potassium, Cushing syndrome, and renal failure.
When used for monitoring, decreasing levels of C-peptide in someone with an insulinoma indicate a response to treatment; levels that are increasing may indicate a tumor recurrence.
A low level of C-peptide is associated with a low level of insulin production. This can occur when insufficient insulin is being produced by the beta cells, with diabetes for example, or when production is suppressed by treatment with exogenous insulin.
Is there anything else I should know?
C-peptide testing is not widely used and may not be available in every laboratory. If a series of C-peptide tests are going to be performed, they should be done at the same laboratory using the same method.
Even though they are produced at the same rate, C-peptide and insulin leave the body by different routes. Insulin is processed and eliminated mostly by the liver, while C-peptide is removed by the kidneys. Since the half-life of C-peptide is about 30 minutes compared to insulin's 5 minutes, normally there will be about 5 times as much C-peptide in the blood as insulin.
What is being tested?
C-peptide is a substance, a short chain of amino acids, that is released into the blood as a byproduct of the formation of insulin by the pancreas. This test measures the amount of C-peptide in a blood or urine sample.
In the pancreas, within specialized cells called beta cells, proinsulin, a biologically inactive molecule, splits apart to form one molecule of C-peptide and one molecule of insulin. Insulin is vital for the transport of glucose into the body's cells and is required on a daily basis. When insulin is required and released from the beta cells into the blood in response to increased levels of glucose, equal amounts of C-peptide are also released. Since C-peptide is produced at the same rate as insulin, it is useful as a marker of insulin production.
In particular, C-peptide testing can be used to help evaluate the production of insulin made by the body (endogenous) and to help differentiate it from insulin that is not produced by the body but is taken in as diabetic medication (exogenous) and so does not generate C-peptide. This test may be done in conjunction with an insulin test.
How is the sample collected for testing?
A blood sample is obtained by inserting a needle into a vein in the arm. If a 24-hour urine sample is required, all urine produced over a 24-hour time period will be collected.
NOTE: If undergoing medical tests makes you or someone you care for anxious, embarrassed, or even difficult to manage, you might consider reading one or more of the following articles: Coping with Test Pain, Discomfort, and Anxiety, Tips on Blood Testing, Tips to Help Children through Their Medical Tests, and Tips to Help the Elderly through Their Medical Tests.
Another article, Follow That Sample, provides a glimpse at the collection and processing of a blood sample and throat culture.
Is any test preparation needed to ensure the quality of the sample?
Fasting for 8 to 10 hours before blood testing is usually required.
- Can I do a C-peptide test at home like I can when I check my blood glucose?
No. The C-peptide test requires special equipment and training to perform.
- If I need to go on the insulin pump, will I need a C-peptide test?
You may. Insulin pumps are usually recommended for those who are not producing sufficient insulin. Sometimes a C-peptide test will be ordered during an initial evaluation to check the status of your beta cells and to see if you are still producing insulin.
- What else is C-peptide used for?
Researchers are starting to better understand the role of C-peptide in the body. Some studies have been conducted to evaluate the use of C-peptide as a therapy for those with diabetes. Results have been promising, showing decreased diabetic complications with improvements in kidney function, blood flow, and nerve function. However, further studies are needed.
© 2017 American Association for Clinical Chemistry, republished from Lab Tests Online.*
Descriptions of clinical laboratory tests were originally prepared for use on Lab Tests Online, an award-winning patient education website on clinical laboratory testing. Lab Tests Online is produced by the American Association for Clinical Chemistry (AACC), a global scientific and medical professional organization dedicated to clinical laboratory science and its application to healthcare. The Lab Tests Online website is developed in collaboration with other laboratory professional societies and is funded in part through corporate sponsorships.