Optimising CYP induction workflows: A miniaturised approach for cost-effective testing

DMDG Early Careers 2026 -- With an aging population and increasing polypharmacy, understanding potential drug-drug interactions (DDIs) for emerging therapies is imperative. Cytochrome P450 (CYP) induction studies are an essential component of the ICH M12 guidelines to determine if a drug has the potential to increase CYP enzyme expression and its ability to cause a DDI. Historically, Labcorp CYP induction experiments have taken place in a 24-well plate format and used traditional RNA extraction techniques. While robust, this was expensive, time consuming and generated a significant amount of plastic waste. The goal of this study was to miniaturise the cytotoxicity and qPCR aspects of a CYP induction assay into a 96-well plate format to increase throughput, reduce costs and reduce the environmental impact of the study.