Feasibility of Adding Volumetric Absorptive Microsampling (VAMS®) Using Mitra® Tips to an Established ELISA Assay to Determine Concentrations of a Monoclonal Antibody in Whole Blood

AAPS PharmSci 360 2025 -- The purpose of these experiments was to take an already existing ligand-binding ELISA assay used for determining concentrations of a monoclonal antibody in human serum and add volumetric absorptive microsampling (VAMS®) to determine concentrations of the same monoclonal antibody in human whole blood. The patient population for the monoclonal antibody was moving into pediatrics, which historically has generated smaller sample volumes for pharmacokinetic (PK) testing. The use of Mitra® tips allows smaller blood volumes to be collected and utilized for PK analysis. The use of VAMS® instead of venous blood sampling provides a more patient-centric approach, allowing the patient to have samples collected at the location most convenient for them, as well as a less-invasive collection.