Development and validation of an LC-MS/MS method for Vitamin A quantification in human serum: A CAP-accredited laboratory study

China Bioanalysis Forum 2025 -- Vitamin A is a fat-soluble vitamin stored in the body that plays a crucial role in various physiological processes, including vision, immune function and cell differentiation. Clinical testing of Vitamin A is crucial for early detection of deficiency, prevention of related diseases, guiding nutritional therapy and monitoring health status. As the results may be used for patient management or safety decisions, the assay must be validated, maintained and reported in accordance with applicable clinical laboratory regulations (CLIA, CAP, UKAS, ISO15189 or relevant local regulation). This study aims to develop and validate a robust liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the accurate and precise quantification of Vitamin A in human serum samples, in accordance with standards set by a College of American Pathologists (CAP)-certified laboratory. The validated method is intended to serve as a reliable and efficient tool to meet the clinical testing requirements for Vitamin A.