Testosterone, Free, Direct With Total Testosterone

CPT: 84402; 84403
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Synonyms

  • Free Testosterone, Direct and Total

Special Instructions

State patient's age and sex on the test request form.

This panel includes Testosterone, Total, which may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R). It is recommended to ask all patients who may be indicated for this test about biotin supplementation. Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.


Expected Turnaround Time

4 - 7 days


Related Documents


Specimen Requirements


Specimen

Serum


Volume

2 mL


Minimum Volume

1 mL (Note: This volume does not allow for repeat testing.)


Container

Red-top tube or gel-barrier tube


Collection

If a red-top tube is used, transfer separated serum to a plastic transport tube.


Storage Instructions

Room temperature


Stability Requirements

Temperature

Period

Room temperature

14 days

Refrigerated

14 days

Frozen

14 days

Freeze/thaw cycles

Stable x3


Causes for Rejection

Gross lipemia (may yield erroneously high free testosterone results); plasma specimen


Test Details


Use

Evaluate hirsutism and masculinization in women; evaluate testicular function in clinical states where the testosterone binding proteins may be altered (obesity, cirrhosis, thyroid disorders). See individual test descriptions for more information.


Limitations

A pediatric reference interval has not been established for the direct free testosterone test.


Methodology

Free: Direct analog enzyme immunoassay (EIA); Total: electrochemiluminescence immunoassay (ECLIA)


LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
140103 Testosterone,Free and Total 58952-3 004227 Testosterone ng/dL 2986-8
140103 Testosterone,Free and Total 58952-3 144981 Free Testosterone(Direct) pg/mL 2991-8

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