11-Desoxycortisol
| 11-Desoxycortisol | | | |
| Number | | 500550 |
| CPT | | 82634 |
Synonyms | | 11-Deoxycortisol; Compound S |
| Special Instructions | | Testing is performed at Esoterix Endocrinology Laboratory: ES#500136. |
| Specimen | | Serum, frozen |
| Volume | | 0.5 mL |
| Minimum Volume | | 0.2 mL (Note: This volume does not allow for repeat testing.) |
| Container | | Red-top tube or gel-barrier tube |
| Collection | | Transfer the serum into a LabCorp PP transpak frozen purple tube with screw cap (LabCorp No 49482). Freeze immediately and maintain frozen until tested. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested. |
| Storage Instructions | | Freeze. Stable for up to 2 years. Stable at room temperature or when refrigerated for up to 24 hours. |
| Use | | Evaluate hypothalamic-pituitary-adrenal axis and pituitary ACTH reserve |
| Methodology | | Liquid chromatography tandem mass spectrometry (LC/MS/MS)
after chromatography and solvent extraction |
| Additional Information | | 11-Deoxycortisol is the immediate precursor of cortisol and follows the same catabolic pathways as cortisol. The conversion of 11-deoxycortisol to cortisol is inhibited by metyrapone, which acts on 11-β-hydroxylase. The metyrapone test (see the Endocrine Appendix ) serves as a reliable and sensitive indicator of pituitary ACTH secretory reserve. The 11-deoxycortisol levels normally increase to 100 times the control value following metyrapone administration. Reduced response occurs in patients with hypoadrenalism or with hypopituitarism and in some patients with diseases of the hypothalamus. Patients with myxedema, some pregnant patients, and those on oral contraceptives respond poorly. |
| References | | Sindler BH, Griffing GT, and Melby JC, et al, “The Superiority of the Metyrapone Test Versus the High-Dose Dexamethasone Test in the Differential Diagnosis of Cushing's Syndrome,” Am J Med, 1983, 74(4):657-62. Spark RF, “Simplified Assessment of Pituitary-Adrenal Reserve: Measurement of Serum 11-Deoxycortisol After Metyrapone,” Ann Intern Med, 1971, 75(5):717-23. |
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