T and B Gene Rearrangement, Southern Blot
| T and B Gene Rearrangement, Southern Blot | | | |
| Number | | 480388 |
| CPT | | 83891; 83892 (x6); 83894 (x2); 83896 (x2); 83897 (x2); 83912 |
| Related Information | | Chronic Lymphocytic Leukemia/Lymphoma Profile, Flow Cytometry Leukemia / Lymphoma Management Test Reference Chart |
| Synonyms | | T- and B-Cell Gene Rearrangements |
| Special Instructions | | This assay is not suitable for skin biopsies, fine-needle aspirates, or formalin-fixed paraffin-embedded tissue, where DNA recovery is usually insufficient. PCR for T- and B-cell clonality [test 480860] should be used instead for these types of specimens. Please state date and time of collection on the request form. For testing on material other than specimen types listed, call the laboratory. Please direct any questions regarding this test to Oncology Customer Service at 800-533-0567. |
| Specimen | | Whole blood, bone marrow, lymph node, or other tissue (specimens may be frozen) |
| Volume | | 14 mL whole blood or 2 mL bone marrow or 0.5-1 g tissue |
| Minimum Volume | | 10 mL whole blood |
| Container | | Lavender-top (EDTA) tube for whole blood or bone marrow, lymph node or tissue in lymph node transport bottle |
| Collection | | Specimen should arrive in the laboratory within 48 hours of collection. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested. |
| Storage Instructions | | Maintain specimen at room temperature. Some specimen types may be submitted frozen; call the laboratory for instructions. |
| Use | | Determine the clonality and lineage of suspected neoplastic lymphoid cell populations by detecting gene rearrangements that occur normally during the maturation of lymphoid cells into T or B lymphocytes |
| Methodology | | Southern blot hybridization |
| References | | Cossman J, Uppenkamp M, Sundeen J, et al, “Molecular Genetics and the Diagnosis of Lymphoma,” Arch Pathol Lab Med, 1998, 112(2):117-27. |
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