T-Cell Gene Rearrangement, Southern Blot
| T-Cell Gene Rearrangement, Southern Blot | | | |
| Number | | 480707 |
| CPT | | 83891; 83892 (x3); 83894 (x2); 83896; 83897; 83912 |
| Related Information | | Chronic Lymphocytic Leukemia/Lymphoma Profile, Flow Cytometry Leukemia / Lymphoma Management Test Reference Chart T-Cell Gene Rearrangement, PCR |
| Synonyms | | TCR Beta Gene Rearrangement by Southern Blot |
| Test Includes | | DNA probe to the T-cell receptor beta-chain constant region |
| Special Instructions | | Please direct any questions regarding this test to Oncology Customer Service at 800-533-0567. |
| Specimen | | Whole blood, bone marrow, fresh or frozen tissue |
| Volume | | 7 mL whole blood or 2 mL bone marrow or 0.5-1 g tissue |
| Minimum Volume | | 3 mL whole blood |
| Container | | Lavender-top (EDTA) tube or yellow-top (ACD-A) tube, tissue in lymph node transport bottle |
| Collection | | Specimen should arrive in the laboratory within 48 hours of collection. Please state the date and time of collection on the request form. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested. |
| Storage Instructions | | Maintain specimen at room temperature. Some specimen types may be submitted frozen; call 800-533-0567 for instructions. |
| Causes for Rejection | | Specimen more than 48 hours old; clotted blood |
| Use | | This assay can be used to detect clonal T-cell gene rearrangements in blood, bone marrow, and tissue specimens. The presence of a monoclonal gene rearrangement usually, but not always, reflects the presence of a T-lymphocytic neoplasm, while polyclonal gene rearrangement patterns are found in benign reactive conditions. Thus, the results of these studies can assist in the diagnosis of lymphoproliferative disorders. |
| Limitations | | The Southern blot analysis may not detect a clonal population if it represents <10% of the total cells in the specimen. The T-Cell Gene Rearrangement by PCR test [480708] can be used to detect smaller populations of malignant cells in blood, bone marrow, and tissue (both fresh and paraffin-embedded). |
| Methodology | | Southern blot analysis |
| References | | Langerak AW, Wolves-Tettero, IL, van Dongen JJ, et al, “Detection of T-Cell Receptor Beta (TCRB) Gene Rearrangement Patterns in T-Cell Malignancies by Southern Blot Analysis,” Leukemia, 1999, 13(6):965-74. |
|