Virus, Direct Detection DFA, Adenovirus
| Virus, Direct Detection DFA, Adenovirus | | | |
| Number | | 185033 |
| CPT | | 87260 |
| Related Information | | Respiratory Syncytial Virus (RSV), Immunoassay Virus Culture, Rapid, Varicella-Zoster Virus (VZV) Virus, Direct Detection DFA, Herpes Simplex Virus |
| Synonyms | | Adenovirus, Direct Detection by DFA |
| Test Includes | | Direct microscopic examination (nonculture) of virus-infected cells. Quality of specimen may be evaluated. |
| Special Instructions | | Submit one specimen per test requested. Specify the exact specimen source/origin (eg, nasopharynx). Indicate a specific test number on the request form. |
| Specimen | | Impression smears of tissues, lesion scrapings and swabs, upper respiratory tract swabs |
| Volume | | Two slides per virus |
| Container | | Plain glass slide, no fixative |
| Collection | | Submit two thinly made air-dried slides. Do not use spray fixative. |
| Storage Instructions | | Refrigerate or maintain specimen at room temperature. |
| Causes for Rejection | | Specimen received after prolonged delay in transport (usually more than 72 hours); unlabeled specimen or name discrepancy between specimen and request label; broken or badly scratched slides; fixative used on slides |
| Reference Interval | | No virus detected |
| Use | | Rapid diagnosis of adenovirus |
| Methodology | | Direct fluorescent antibody (DFA) |
| Additional Information | | Generally, this test is not as sensitive as cell culture. |
| References | | Drew WL, “Controversies in Viral Diagnosis,” Rev Infect Dis, 1986, 8(5):814-24 (review). Drew WL, “Diagnostic Virology,” Clin Lab Med, 1987, 7(4):721-40 (review). Smith TF, “Rapid Methods for the Diagnosis of Viral Infections,” Lab Med, 1987, 18:16-20. |
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