Antinuclear Antibodies (ANA) Comprehensive Profile

Antinuclear Antibodies (ANA) Comprehensive Profile

Number
	165092
CPT
	86225; 86235 (x8)
Synonyms
	ANA ; Centromere B ; dsDNA ; ENA ; Histone ; Sjögren ; SLE
Test Includes
	Antinuclear antibodies (ANA); centromere B; dsDNA; ENA; histone; Sjögren; systemic lupus erythematosus (SLE)
Specimen
	Serum
Volume
	1 mL
Minimum Volume
	0.5 mL (Note: This volume does not allow for repeat testing.)
Container
	Red-top tube or gel-barrier tube
Storage Instructions
	Maintain specimen at room temperature.
Causes for Rejection
	Hemolysis; lipemia; gross bacterial contamination; icterus
Use
	Detect antibodies associated with SLE and mixed connective tissue disease. Anti-Sm is highly specific for SLE. Anti-RNP is found with a variety of rheumatoid diseases. RNP, Sm, SS-A, SS-B, Scl-70, and Jo-1 are extractable nucleoprotein complexes found in the nucleus and/or cytoplasm of substrate cells.
Methodology
	Multiplex bead flow cytometry
References
	Kopnitsky M and Meenan J, “AtheNA Multilyte ANA Test System: The Next Generation of Antinuclear Testing,” Clin Lab Products, 2002, 31:38-49. Tan EM, Chan EK, Sullivan KF, et al, “Antinuclear Antibodies (ANAs): Diagnostically Specific Immune Markers and Clues Toward the Understanding of Systemic Autoimmunity,” Clin Immunol Immunopathol, 1988, 47(2):121-41. von Muhlen CA and Nakamura RM, “Clinical and Laboratory Evaluation of Systemic Rheumatic Diseases,” Clinical Diagnosis and Management by Laboratory Methods, 20th ed, Henry JB, ed, Philadelphia PA: WB Sanders Co, 2001, 974-89. von Muhlen CA and Tan EM, “Autoantibodies in the Diagnosis of Systemic Rheumatic Diseases,” Semin Arthritis Rheum, 1995, 24(5):323-58.