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Helper Suppressor Ratio, CD4:CD8, Pediatric

CPT: 86360
Updated on 12/9/2019
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Test Includes

Percentage CD4+; absolute CD4+; percentage CD8+; absolute CD8+; CD4:CD8 ratio; CBC with differential


Special Instructions

State date and time of collection on the test request form. For Pediatric samples.

This assay is currently not available in New York state.

State date and time of collection on the test request form. For Pediatric samples; not available in New York State.

State date and time of collection on the test request form. For Pediatric samples.

This assay is currently not available in New York state.


Expected Turnaround Time

1 - 3 days


Related Documents


Specimen Requirements


Specimen

Whole blood


Volume

1 mL


Minimum Volume

0.5 mL


Container

BD EDTA Microtainer, BD EDTA 2.0 mL Vacutainer tube or BD EDTA 4.0 mL Vacutainer tube


Collection

Invert tube 8 to 10 times immediately after collection. To preserve cellular viability, collect specimen so it will arrive in the laboratory within 48 hours of collection. Indicate date and time of venipuncture on the tube(s) and on the request form.


Storage Instructions

Maintain specimen at room temperature.


Stability Requirements

Temperature

Period

Room temperature

2 days

Refrigerated

Unstable

Frozen

Unstable

Freeze/thaw cycles

Unstable


Causes for Rejection

Hemolysis; specimen refrigerated or frozen; clotted specimen; contaminated specimen


Test Details


Use

HIV-1 infection results in a decrease of CD4 T cells, an increase of CD8 T cells, a decrease in the CD4:CD8 ratio, and a progressive destruction of immune function. In HIV-1 seropositive patients, enumeration of CD4 T cells may be used for prognostic purposes and to monitor disease progression and antiretroviral therapy.


Methodology

Flow cytometry


References

1997 revised guidelines for performing CD4+ T-cell determinations in persons infected with human immunodeficiency virus (HIV). Centers for Disease Control and Prevention. MMWR Recomm Rep. 1997 Jan 10;46(RR-2):1-29.9011774
Giorgi J, Kesson A, Chou CC. Immunodeficiency and infectious diseases. In: Rose N, deMacario E, Fahey J, et al, eds. Manual of Clinical Laboratory Immunology. 4th ed. Washington, DC: ASM Press;1992:chap 25.
Stevens RA, Lempicki RA, Natarajan V, et al. General immunologic evaluation of patients with immunodeficiency virus infection. In: Detrick B, Hamilton RG, Folds JD, et al, eds. Manual of Molecular and Clinical Laboratory Immunology. 7th ed. Washington, DC: ASM Press;2006:chap 95.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
505300 CD4/CD8 Pediatric 65758-5 505010 Absolute CD 4 Helper /uL 24467-3
505300 CD4/CD8 Pediatric 65758-5 505009 % CD 4 Pos. Lymph. % 8123-2
505300 CD4/CD8 Pediatric 65758-5 505579 Abs. CD 8 Suppressor /uL 14135-8
505300 CD4/CD8 Pediatric 65758-5 505580 % CD 8 Pos. Lymph. % 8101-8
505300 CD4/CD8 Pediatric 65758-5 505273 CD4/CD8 Ratio 54218-3
505300 CD4/CD8 Pediatric 65758-5 005025 WBC x10E3/uL 6690-2
505300 CD4/CD8 Pediatric 65758-5 005033 RBC x10E6/uL 789-8
505300 CD4/CD8 Pediatric 65758-5 005041 Hemoglobin g/dL 718-7
505300 CD4/CD8 Pediatric 65758-5 005058 Hematocrit % 4544-3
505300 CD4/CD8 Pediatric 65758-5 015065 MCV fL 787-2
505300 CD4/CD8 Pediatric 65758-5 015073 MCH pg 785-6
505300 CD4/CD8 Pediatric 65758-5 015081 MCHC g/dL 786-4
505300 CD4/CD8 Pediatric 65758-5 105007 RDW % 788-0
505300 CD4/CD8 Pediatric 65758-5 015172 Platelets x10E3/uL 777-3
505300 CD4/CD8 Pediatric 65758-5 015107 Neutrophils % 770-8
505300 CD4/CD8 Pediatric 65758-5 015123 Lymphs % 736-9
505300 CD4/CD8 Pediatric 65758-5 015131 Monocytes % 5905-5
505300 CD4/CD8 Pediatric 65758-5 015149 Eos % 713-8
505300 CD4/CD8 Pediatric 65758-5 015156 Basos % 706-2
505300 CD4/CD8 Pediatric 65758-5 115398 Immature Cells N/A
505300 CD4/CD8 Pediatric 65758-5 015909 Neutrophils (Absolute) x10E3/uL 751-8
505300 CD4/CD8 Pediatric 65758-5 015917 Lymphs (Absolute) x10E3/uL 731-0
505300 CD4/CD8 Pediatric 65758-5 015925 Monocytes(Absolute) x10E3/uL 742-7
505300 CD4/CD8 Pediatric 65758-5 015933 Eos (Absolute) x10E3/uL 711-2
505300 CD4/CD8 Pediatric 65758-5 015941 Baso (Absolute) x10E3/uL 704-7
505300 CD4/CD8 Pediatric 65758-5 015108 Immature Granulocytes % 71695-1
505300 CD4/CD8 Pediatric 65758-5 015911 Immature Grans (Abs) x10E3/uL 53115-2
505300 CD4/CD8 Pediatric 65758-5 015945 NRBC % 58413-6
505300 CD4/CD8 Pediatric 65758-5 015180 Hematology Comments: 18314-5
Reflex Table for Immature Cells
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115399 Immature Cells 115400 Bands % 35332-6
Reflex Table for Immature Cells
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115399 Immature Cells 115401 Metamyelocytes % 28541-1
Reflex Table for Immature Cells
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115399 Immature Cells 115403 Myelocytes % 26498-6
Reflex Table for Immature Cells
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115399 Immature Cells 115404 Promyelocytes % 26498-6
Reflex Table for Immature Cells
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115399 Immature Cells 115405 Blasts/blast like cells % 26498-6
Reflex Table for Immature Cells
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115399 Immature Cells 115406 Megakaryocytes % 19252-6
Reflex Table for Immature Cells
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 115399 Immature Cells 115265 Other, Lineage Uncertain % 55433-7

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CPT Statement/Profile Statement

The LOINC® codes are copyright © 1994-2018, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf