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Human Immunodeficiency Virus 1 (HIV-1), Qualitative, RNA

CPT: 87535
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Expected Turnaround Time

2 - 5 days


Related Documents

For more information, please view the literature below.

HIV Specimen Collection Guide


Specimen Requirements


Specimen

Plasma or serum


Volume

1 mL


Minimum Volume

0.5 mL


Container

Lavender-top (EDTA) tube, yellow-top (ACD) tube, red-top tube, or gel-barrier tube


Collection

Collect specimen in lavender-top (EDTA), yellow-top (ACD), red-top tube, or gel-barrier tube. Whole blood specimens stored at room temperature should be centrifuged within 72 hours of collection. Transfer serum or plasma to a polypropylene screw-cap tube for shipment.


Storage Instructions

Sample may be stored for 72 hours at room temperature. It is recommended that serum or plasma be refrigerated or frozen for shipment.


Stability Requirements

Temperature

Period

Room temperature

3 days

Refrigerated

14 days

Frozen

6 months

Freeze/thaw cycles

Stable x5


Causes for Rejection

Specimen grossly hemolyzed; received outside of specimen and/or storage requirements


Test Details


Use

This test is intended for use as an aid in the diagnosis of HIV-1 infection, including acute or primary infection. Presence of HIV-1 RNA in the plasma of patients without antibodies to HIV-1 is indicative of acute or primary HIV-1 infection. This assay may also be used as an additional test, when it is reactive, to confirm HIV-1 infection in an individual whose specimen is repeatedly reactive for HIV-1 antibodies. This assay is not intended for use in donor testing.


Methodology

Transcription-mediated amplification (TMA)


LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
139350 RNA Qualitative 25835-0 139351 HIV-1 RNA Qualitative 25835-0

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