Myeloperoxidase (MPO)

CPT: 83876
Updated on 5/21/2019
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Synonyms

  • MPO

Related Documents


Specimen Requirements


Specimen

Plasma


Volume

0.5 mL


Minimum Volume

0.2 mL


Container

Lavender-top (EDTA) tube or green-top (heparin) tube


Collection

Collect whole blood using venipuncture techniques. Gently mix the blood with the anticoagulant by inverting sample tube several times (do not shake).

Place freshly collected blood samples on ice or in a refrigerator 2°C to 8°C immediately, and store them at 2°C to 8°C until separation. Plasma should be physically separated from cells within two hours of collection by centrifugation at 2°C to 8°C.

Special precaution needs to be taken to ensure transfer of the plasma layer to a polypropylene (not glass) tube while avoiding carryover of any red blood cells or buffy coat white cells. The plasma samples thus prepared may be refrigerated at 2°C to 8°C for five days.

If plasma samples are prepared by gel based vacuum tubes, the plasma samples need to be transferred to separated tubes from the top of the gels immediately after the centrifugation.

Increased levels of MPO may be observed if the plasma samples are left on the top of the gels for more than eight hours before transferring to separate tubes.

Collect whole blood using venipuncture techniques. Gently mix the blood with the anticoagulant by inverting sample tube several times (do not shake).

Place freshly collected blood samples on ice or in a refrigerator 2°C to 8°C immediately, and store them at 2°C to 8°C until separation. Plasma should be physically separated from cells within two hours of collection by centrifugation at 2°C to 8°C.

Special precaution needs to be taken to ensure transfer of the plasma layer to a polypropylene (not glass) tube while avoiding carryover of any red blood cells or buffy coat white cells. The plasma samples thus prepared may be refrigerated at 2°C to 8°C) for five days.

If plasma samples are prepared by gel based vacuum tubes, the plasma samples need to be transferred to separated tubes from the top of the gels immediately after the centrifugation.

Increased levels of MPO may be observed if the plasma samples are left on the top of the gels for more than eight hours before transferring to separate tubes.

Collect whole blood using venipuncture techniques. Gently mix the blood with the anticoagulant by inverting sample tube several times (do not shake).

Place freshly collected blood samples on ice or in a refrigerator 2°C to 8°C immediately, and store them at 2°C to 8°C until separation. Plasma should be physically separated from cells within two hours of collection by centrifugation at 2°C to 8°C.

Special precaution needs to be taken to ensure transfer of the plasma layer to a polypropylene (not glass) tube while avoiding carryover of any red blood cells or buffy coat white cells. The plasma samples thus prepared may be refrigerated at 2°C to 8°C for five days.

If plasma samples are prepared by gel based vacuum tubes, the plasma samples need to be transferred to separated tubes from the top of the gels immediately after the centrifugation.

Increased levels of MPO may be observed if the plasma samples are left on the top of the gels for more than eight hours before transferring to separate tubes.


Storage Instructions

Refrigerate


Stability Requirements

Temperature

Period

Refrigerated

5 days (stability determined by manufacturer or literature reference)

Frozen

6 months (stability determined by manufacturer or literature reference)

Temperature

Period

Refrigerated

5 days (stability determined by manufacturer or literature reference)

Frozen

6 months (stability determined by manufacturer or literature reference)


Causes for Rejection

Specimen type other than heparinized plasma or EDTA plasma; plasma samples left on top of gels for more than eight hours before transferring to separate tubes; specimen received at room temperature

Specimen type other than heparinized plasma or EDTA plasma; plasma samples left on top of gels for more than eight hours before transferring to separate tubes

Specimen type other than heparinized plasma or EDTA plasma; plasma samples left on top of gels for more than eight hours before transferring to separate tubes; specimen received at room temperature


Test Details


Use

Elevated levels of plasma MPO are a sensitive indicator of inflammatory disorders.


Limitations

Erratic results can be observed if plasma is not centrifuged and separated from red cells immediately.

Results of this test are labeled for "research purposes only." The result should not be used for treatment or for diagnostic purposes without confirmation of the diagnosis by another medically established diagnostic product or procedure. The performance characteristics were determined by LabCorp.

Erratic results can be observed if plasma is not centrifuged and separated from red cells immediately.

Results of this test are labeled for research purposes only by the assay's manufacturer. The performance characteristics of this assay have not been established by the manufacturer. The result should not be used for treatment or for diagnostic purposes without confirmation of the diagnosis by another medically established diagnostic product or procedure. The performance characteristics were determined by LabCorp.

Erratic results can be observed if plasma is not centrifuged and separated from red cells immediately.

Results of this test are labeled for "research purposes only." The result should not be used for treatment or for diagnostic purposes without confirmation of the diagnosis by another medically established diagnostic product or procedure. The performance characteristics were determined by LabCorp.


Methodology

Latex enhanced immunoturbidimetric method


Reference Interval

• Low CVD Risk: <470 pmol/L

• Moderate Risk: 470–539 pmol/L

• High Risk: >539 pmol/L


Additional Information

MPO plays an important role in the innate host-defense mechanism of human and animals.

Myeloperoxidate (MPO) is a hemoprotein present in leukocytes and catalyzes the hydrogen peroxide mediated peroxidation of halide ions to produce strong reactive oxidant species such as hypochlorous acid that are of potent antimicrobial activities against a broad range of invading parasites and pathogens. However, MPO-derived reactive oxidants also promote host tissue injury through lipid and protein peroxidations that lead to systemic inflammation.


References

Nilsson L, Brunnkvist S, Nilsson U, et al. Activation of inflammatory systems during cardiopulmonary bypass. Scand J Thorac Cardiovasc Surg. 1988;22(1):51-53.2838898
Heinecke JW. Mechanisms of oxidative damage by myeloperoxidase in atherosclerosis and other inflammatory disorders. J Lab Clin Med. 1999 Apr;133(4):321-325.10218761
Podil'chak MD, Terletskaia LM. Clinical value of determining myeloperoxidase and alkaline phosphatase activity of the leukocytes in patients with suppurative-inflammatory processes. Klin Khir. 1988;(1):59-60.2835543
Re G, Azzimondi G, Lanzarini C, Bassein L, Vaona I, Guarnieri C. Plasma lipoperoxidative markers in ischaemic stroke suggest brain embolism. Eur J Emerg Med. 1997 Mar;4(1):5-9.9152688
Biasucci LM, D'Onofrio G, Liuzzo G, et al. Intracellular neutrophil myeloperoxidase is reduced in unstable angina and acute myocardial infarction, but its reduction is not related to ischemia. J Am Coll Cardiol. 1996 Mar 1;27(3):611-616.8606272
Shih J, Datwyler SA, Hsu SC, et al. Effect of collection tube type and preanalytical handling on myeloperoxidase concentrations. Clin Chem. 2008 Jun;54(6):1076-1079.18509013

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
123006 Myeloperoxidase (MPO) 66853-3 123007 Myeloperoxidase (MPO) pmol/L 66853-3

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