Hepatitis B Virus (HBV), Quantitative, DNA (Abbott® RealTime)

Hepatitis B Virus (HBV), Quantitative, DNA (Abbott RealTime)
Hepatitis B Virus (HBV), Quantitative, DNA (Abbott® RealTime)
CPT: 87517
Updated on 04/21/2024
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Expected Turnaround Time

4 - 7 days


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Specimen Requirements


Specimen

Serum or plasma

Serum or plasma (EDTA)

Serum or plasma


Volume

2 mL

3 mL

2 mL


Minimum Volume

1 mL (Note: This volume does not allow for repeat testing.)


Container

Ship in a screw-cap polypropylene frozen transport tube, centrifuged serum separator tube (SST) or centrifuged PPT™ tubes.

Ship in a screw-cap polypropylene frozen transport tube, centrifuged serum separator tube (SST) or centrifuged PPT™ tubes.


Collection

Collect specimen in red-top, serum separator (SST), lavender-top (EDTA), PPT™ or yellow-top (ACD-A) tubes. Centrifuge specimen. If drawn in a red-top, EDTA or ACD, the serum or plasma must be transferred to a screw-cap polypropylene transport tube and frozen. Whole blood specimen may be held at room temperature for 24 hours or or refrigerated for 72 hours prior to centrifugation. Serum and plasma specimens may be stored at room temperature for 20 hours, refrigerated 72 hours, frozen at -20°C for 60 days or -70°C long term. Ship frozen on dry ice. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate specimens for each test requested.

Freshly drawn specimens (whole blood) may be held at 2° C to 30° C for up to 6 hours prior to centrifugation. Remove serum or plasma, transfer to a screw-cap polypropylene transport tube. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate specimens for each test requested.

Collect specimen in red-top, serum separator (SST), lavender-top (EDTA), PPT™ or yellow-top (ACD-A) tubes. Centrifuge specimen. If drawn in a red-top, EDTA or ACD, the serum or plasma must be transferred to a screw-cap polypropylene transport tube and frozen. Whole blood specimen may be held at room temperature for 24 hours or or refrigerated for 72 hours prior to centrifugation. Serum and plasma specimens may be stored at room temperature for 20 hours, refrigerated 72 hours, frozen at -20°C for 60 days or -70°C long term. Ship frozen on dry ice. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate specimens for each test requested.


Storage Instructions

Freeze.

Freeze. Stable at room temperature for six hours (whole blood) or one day after centrifugation. Stable refrigerated for up to 3 days after centrifugation. Freeze for long term storage.

Freeze..


Stability Requirements

Temperature

Period

Room temperature

20 hours (stability provided by manufacturer or literature reference)

Refrigerated

72 hours (stability provided by manufacturer or literature reference)

Combined room temperature and refrigerated storage times must not exceed 72 hours.

Frozen

-20°C for up to 60 days (stability provided by manufacturer or literature reference)

-70°C long term (stability provided by manufacturer or literature reference)

Freeze/thaw cycles

Stable x1

Temperature

Period

Room temperature

20 hours (stability provided by manufacturer or literature reference)

Refrigerated

72 hours (stability provided by manufacturer or literature reference)

Combined room temperature and refrigerated storage times must not exceed 72 hours.

Frozen

-20°C for up to 60 days (stability provided by manufacturer or literature reference)

-70°C long term (stability provided by manufacturer or literature reference)

Freeze/thaw cycles

Stable x1


Causes for Rejection

Hemolysis; plasma other than EDTA or ACD-A; specimen received in a "pop-top" or "snap-cap" tube; quantity not sufficient for analysis; whole blood; frozen glass tubes

Hemolysis; heparinized plasma; nonfrozen specimens; specimen received in "pop-top" or "snap-cap" tube; whole blood

Hemolysis; plasma other than EDTA or ACD-A; specimen received in a "pop-top" or "snap-cap" tube; quantity not sufficient for analysis; whole blood; frozen glass tubes


Test Details


Use

This test is used to determine the number of international units (IU) of hepatitis B virus DNA per milliliter of serum or plasma in known HBV-positive patients.

Determine the number of international units (IU) of hepatitis B virus DNA per milliliter of serum or plasma in known HBV-positive patients.

This test is used to determine the number of international units (IU) of hepatitis B virus DNA per milliliter of serum or plasma in known HBV-positive patients.


Limitations

Assay range is 10 IU/mL to 1,000,000,000 IU/mL


Methodology

Abbott Alinity m HBV assay

Abbott m2000 RealTime HBV assay

Abbott Alinity m HBV assay


LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
551745 HBV DNA RealTime Abbott 95147-5 551746 Hepatitis B Quantitation IU/mL 42595-9
551745 HBV DNA RealTime Abbott 95147-5 551749 HBV log10 log10 IU/mL 45161-7
551745 HBV DNA RealTime Abbott 95147-5 551758 Test Information: N/A
Reflex Table for Hepatitis B Quantitation
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 551753 HBV DNA (International Units) 551756 HBV DNA (International Units) IU/mL 42595-9
Reflex Table for Hepatitis B Quantitation
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 551753 HBV DNA (International Units) 551757 HBV Log10 log10 IU/mL 48398-2

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