Microarray-Products of Conception (POC) Reveal® FFPE

CPT: 81229
Updated on 9/22/2019
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Synonyms

  • aCGH
  • CGH
  • CMA
  • Microarray
  • Reveal® SNP Microarray-Products of Conception (POC)
  • SNP Array
  • WGA

Special Instructions

Pertinent medical findings should accompany the test request form. Please direct questions to genetic customer service at 800-345-4363.

There may be an additional charge for formalin positive tissue samples requiring embedding and pathology.

Pertinent medical findings should accompany the test request form. Please direct questions to genetic customer service at 800-345-4363.

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Pertinent medical findings should accompany the test request form. Please direct questions to genetic customer service at 800-345-4363.

<p>There may be an additional charge for formalin positive tissue samples requiring embedding and pathology.

Expected Turnaround Time

14 - 21 days


Related Documents


Specimen Requirements


Specimen

Preferred fixed products of conception (POC), formalin-fixed paraffin-embedded (FFPE) tissue


Volume

One paraffin block or ten 10-micron thick section slides


Minimum Volume

Extracted DNA from received specimen must yield at least 10 ul at a concentration of 8 ng/ul.

Sufficient sample to obtain enough DNA to run microarray

Extracted DNA from received specimen must yield at least 10 ul at a concentration of 8 ng/ul.


Container

Paraffin block transport pouch


Collection

Specimen should be fixed in 10% neutral-buffered formalin or formaldehyde.


Storage Instructions

Room temperature


Causes for Rejection

No fetal visualized on pathology report; only a small area is identified on fixed sample as fetal, and is less than 50% of sample

Improperly embedded preparation

No fetal visualized on pathology report; only a small area is identified on fixed sample as fetal, and is less than 50% of sample


Test Details


Use

This test will detect chromosomal imbalance that could be associated with developmental delay/congenital anomalies.It provides detection of uniparental disomy of any chromosome, the percent and location of homozygosity, including the degree of identity by descent.It will also allow the detection of complete or partial molar pregnancies.

Detects chromosomal imbalance that may be associated with fetal loss and is ideal for detection of complete or partial molar pregnancies.

The microarray will detect DNA copy-number changes at a threshold >1Mb for deletions, >2 Mb for duplications. Also will detect copy-neutral changes associated with uniparental disomy and identity by descent.

This test will detect chromosomal imbalance that could be associated with developmental delay/congenital anomalies.It provides detection of uniparental disomy of any chromosome, the percent and location of homozygosity, including the degree of identity by descent.It will also allow the detection of complete or partial molar pregnancies.


Limitations

This SNP assay does not detect balanced rearrangements, low-level mosaicism (<10%), marker chromosomes that only contain heterochromatin, or tetraploidy. Genotypes in some formalin-fixed tissues are unable to be determined, allowing only genomic dosage to be determined.

This test was developed and its performance characteristics determined by LabCorp. It has not been cleared or approved by the Food and Drug Administration.

This assay does not detect balanced rearrangements and low level mosaicism, or tetraploidy.

This test was developed, and its performance characteristics determined, by LabCorp. It has not been cleared or approved by the US Food and Drug Administration (FDA).

This SNP assay does not detect balanced rearrangements, low-level mosaicism (<10%), marker chromosomes that only contain heterochromatin, or tetraploidy. Genotypes in some formalin-fixed tissues are unable to be determined, allowing only genomic dosage to be determined.

This test was developed and its performance characteristics determined by LabCorp. It has not been cleared or approved by the Food and Drug Administration.


Methodology

SNP Microarray analysis is performed using the Oncoscan ® FFPE platform, which uses more than 220,000 SNP probes with a median spacing of 5.0kb, within the majority of genes.

Whole genome SNP copy number microarray analysis using Molecular Inversion Probe (MIP) technology optimized for highly degraded FFPE samples.

SNP Microarray analysis is performed using the Oncoscan ® FFPE platform, which uses more than 220,000 SNP probes with a median spacing of 5.0kb, within the majority of genes.


References

Wang Y, Cheng Q, Meng L, et al. Clinical application of SNP array analysis in first-trimester pregnancy loss: a prospective study. Clin Genet. 2017 Jun;91(6):849-858.27883173

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
511997 Reveal(R) POC Paraffin 511998 Specimen Type 31208-2
511997 Reveal(R) POC Paraffin 511999 # of Genotyping Targets N/A
511997 Reveal(R) POC Paraffin 512000 Array Type N/A
511997 Reveal(R) POC Paraffin 512001 Diagnosis 48000-4
511997 Reveal(R) POC Paraffin 512002 Interpretation 62365-2
511997 Reveal(R) POC Paraffin 512003 Director Review 48672-0
511997 Reveal(R) POC Paraffin 512123 PDF 80563-0

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