Antinuclear Antibodies (ANA) by IFA, Reflex to 9-biomarker profile, dsDNA, RNP, Sm, SS-A, SS-B, Scl-70, Chromatin, Jo-1, Centromere B by Multiplex Immunoassay

Antinuclear Antibodies (ANA) by IFA, Reflex to dsDNA, RNP, Sm, SS-A, SS-B, Scl-70, Chromatin, Jo-1, Centromere B

CPT: 86038
Updated on 11/26/2018
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Test Details

Synonyms

  • ANA IFA Reflex

Test Includes

Detect antibodies to nuclear antigens by IFA with patterns and titers with reflex if positive to centromere B; chromatin; dsDNA; Jo-1; RNP; scl-70; Sjögren's A; Sjögren's B; Smith (Sm). If reflex testing is performed, additional charges/CPT code(s) may apply.

Use

Assay for screening for ANAs in patients suspected of having connective tissue disease.

Limitations

Males and females older than 80 years of age have a 50% incidence of low titer ANA. Various medications can induce a "lupoid" condition and elevated ANA titers. Usually the titer decreases following removal of the drug. Low antibody levels may be found in diseases other than connective tissue disease.

Methodology

Indirect immunofluorescence assay (IFA) and multiplex flow immunoassay

Additional Information

The indirect immunofluorescent test has three elements to consider in the result:

1. Positive or negative fluorescence. A negative test is strong evidence against a diagnosis of SLE but not conclusive. See Anti-DNA (Single-stranded) Antibodies, Quantitative, IgG [161422].

2. The titer (dilution) to which fluorescence remains positive (provides a reflection of the concentration or avidity of the antibody). Many individuals, particularly the elderly, may have low titer ANA without significant disease substantiated after work-up.

3. The pattern of nuclear fluorescence (reflecting specificity for various diseases).

Cytoplasmic (non-nuclear) staining patterns may also be noted with the IFA methodology.

Multiplex ANA detects up to 11 specific antibodies of the 100+ antibodies that may be found in the ANA IFA.

Specimen Requirements

Specimen

Serum

Volume

1 mL

Minimum Volume

0.5 mL

Container

Red-top tube or gel-barrier tube

Storage Instructions

Room temperature

Stability Requirements

Temperature

Period

Room temperature

7 days

Refrigerated

7 days

Frozen

7 days

Freeze/thaw cycles

Stable x2

Causes for Rejection

Hemolysis; lipemia; gross bacterial contamination

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
340897 ANA 5048-4 164947 Antinuclear Antibodies, IFA 5048-4
340897 ANA 5048-4 164945 Please Note: 77202-0
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 012625 FANA Staining Patterns 016279 Homogeneous Pattern 20398-4
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 012625 FANA Staining Patterns 016287 Nucleolar Pattern 20399-2
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 012625 FANA Staining Patterns 016295 Speckled Pattern 20401-6
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 012625 FANA Staining Patterns 016303 Centromere Pattern 5077-3
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 012625 FANA Staining Patterns 016264 Spindle Apparatus Pattern 53003-0
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 012625 FANA Staining Patterns 016265 Nuclear Membrane Pattern 54006-2
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 012625 FANA Staining Patterns 016266 Midbody Pattern 54155-7
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 012625 FANA Staining Patterns 016267 Nuclear Dot Pattern 53998-1
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 012625 FANA Staining Patterns 016268 PCNA Pattern 53006-3
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 012625 FANA Staining Patterns 016269 Centriole Pattern 54151-6
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 012625 FANA Staining Patterns 016271 Note: N/A
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 016279 Homogeneous Pattern 20398-4
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 016287 Nucleolar Pattern 20399-2
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 016295 Speckled Pattern 20401-6
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 016303 Centromere Pattern 5077-3
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 016264 Spindle Apparatus Pattern 53003-0
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 016265 Nuclear Membrane Pattern 54006-2
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 016266 Midbody Pattern 54155-7
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 016267 Nuclear Dot Pattern 53998-1
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 016268 PCNA Pattern 53006-3
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 016269 Centriole Pattern 54151-6
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 016271 Note: N/A
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 096343 Anti-DNA (DS) Ab Qn IU/mL 5130-0
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 016354 RNP Antibodies AI 29374-6
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 016362 Smith Antibodies AI 11090-8
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 018707 Antiscleroderma-70 Antibodies AI 27416-7
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 012684 Sjogren's Anti-SS-A AI 17792-3
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 012692 Sjogren's Anti-SS-B AI 17791-5
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 012581 Antichromatin Antibodies AI 51775-5
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 161457 Anti-Jo-1 AI 11565-9
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 164816 Anti-Centromere B Antibodies AI 53982-5
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 164869 See below: N/A
Reflex Table for Antinuclear Antibodies, IFA
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 160024 ENA+DNA/DS+Antich+Centro+FA... 164949 ANA IFA Tracking to BioPlex N/A

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