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Testosterone, Free, Direct With Total Testosterone

TEST: 140103
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CPT: 84402; 84403
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Test Details

Synonyms

  • Free Testosterone, Direct and Total

Use

Evaluate hirsutism and masculinization in women; evaluate testicular function in clinical states where the testosterone binding proteins may be altered (obesity, cirrhosis, thyroid disorders). See individual test descriptions for more information.

Limitations

A pediatric reference interval has not been established for the direct free testosterone test.

Methodology

Free: Direct analog enzyme immunoassay (EIA); Total: electrochemiluminescence immunoassay (ECLIA)

Related Documents

For more information, please view the literature below.

Frequently Asked Questions: Testosterone by Mass Spectrometry—New Profiles

Testosterone: Aiding in the Diagnosis of Androgen Dysfunction and Hypogonadism

Testosterone Laboratory Update

Specimen Requirements

Specimen

Serum

Volume

2 mL

Minimum Volume

0.7 mL (Note: This volume does not allow for repeat testing.)

Container

Red-top tube or gel-barrier tube

Collection

If a red-top tube is used, transfer separated serum to a plastic transport tube.

Storage Instructions

Room temperature

Stability Requirements

Temperature

Period

Room temperature

14 days

Refrigerated

14 days

Frozen

14 days

Freeze/thaw cycles

Stable x3

Causes for Rejection

Gross lipemia (may yield erroneously high free testosterone results); plasma specimen

Clinical Information

Special Instructions

State patient's age and sex on the test request form.

This panel includes Testosterone, Total, which may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R). It is recommended to ask all patients who may be indicated for this test about biotin supplementation. Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
140103 Testosterone,Free and Total 004227 Testosterone, Serum ng/dL 2986-8
140103 Testosterone,Free and Total 144981 Free Testosterone(Direct) pg/mL 2991-8

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CPT Statement/Profile Statement

The LOINC® codes are copyright © 1994-2018, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf