T-Cell Receptor β-Chain Gene Rearrangements

CPT: 81340
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Test Details


  • TCRB Gene Clonality Assay


This assay can be used to detect clonal T-cell receptor β-chain gene rearrangements in blood, bone marrow, and tissue specimens. The presence of a monoclonal gene rearrangement usually, but not always, reflects the presence of a T-lymphocytic neoplasm, while polyclonal gene rearrangement patterns are found in benign reactive conditions. Thus, the results of these studies can assist in the diagnosis of lymphoproliferative disorders.


This PCR assay is able to detect a clonal DNA population at the sensitivity of 5% in a background of polyclonal DNA.


Polymerase chain reaction (PCR)

Specimen Requirements


Whole blood, bone marrow, formalin-fixed, paraffin-embedded (FFPE) tissue block or slides, fresh or frozen tissue


3 to 5 mL whole blood, 1 mL bone marrow, five unstained slides at 10 μM or formalin-fixed, paraffin-embedded tissue block, tissue in RPMI or frozen.

Minimum Volume

0.5 mL whole blood, 0.5 mL bone marrow, two unstained slides at 10 μM


Lavender-top (EDTA) or green-top (heparin) tube, slides, blocks, tissue in RPMI, or frozen


Fresh tissue should be placed in RPMI medium or shipped frozen on dry ice. Also acceptable are small samples (eg, punch biopsies, fine-needle aspirate).

Storage Instructions

Maintain blood, bone marrow or tissue in RPMI at 2°C to 8°C, FFPE specimens at room temperature, frozen tissue at -80°C

Causes for Rejection

Frozen whole blood/bone marrow; tumor block containing no tumor tissue; broken or stained slides; quantity not sufficient for analysis

Clinical Information

Special Instructions

Please direct any questions regarding this test to customer service at 800-345-4363.


van Krieken JH, Langerak AW, Macintyre EA, et al. Improved reliability of lymphoma diagnostics via PCR-based clonality testing: Report of the BIOMED-2 concerted action BHM4-CT98-3936. Leukemia. 2007 Feb; 21(2): 201-206.17170732


Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
480985 TCRB Gene Clonality 480986 TCRB Gene Clonality Results: 21751-3
480985 TCRB Gene Clonality 480987 Clonal Size: N/A
480985 TCRB Gene Clonality 480989 Block Number: N/A
480985 TCRB Gene Clonality 480993 Director Review: 72486-4
480985 TCRB Gene Clonality 480984 Methodology: 49549-9
480985 TCRB Gene Clonality 480994 References: N/A

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